Mouse Ace ELISA Kit

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  • Alternative name

    Mouse ACE ELISA Kit;Mouse dipeptidyl carboxypeptidase I ELISA Kit;Mouse kininase II ELISA Kit;Mouse Dcp1 ELISA Kit;Mouse AW208573 ELISA Kit;Mouse CD143 ELISA Kit;Mouse angiotensin I converting enzyme (peptidyl-dipeptidase A) 1 ELISA Kit;Mouse angiotensin-converting enzyme ELISA Kit;Mouse dipeptidyl peptidase ELISA Kit;

  • Catalog
    E042376
  • species
    Mouse
  • GeneAce
  • Other Species Human ACE ELISA KitHuman ACE I ELISA KitHuman ACE II ELISA KitMouse ACE I ELISA KitMouse ACE II ELISA KitBovine ACE ELISA KitChicken ACE ELISA KitRabbit ACE ELISA KitRat Ace ELISA Kit
  • SpecificityNatural and recombinant mouse ACE
  • SamplesSerum, Plasma , tissue homogenates,Cell culture supernates,Other biological fluids.
  • Sensitivity< 10pg/ml
  • Intended UseMouse Ace ELISA Kit allows for the in vitro quantitative determination of Ace , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
  • StorageStore the whole ELISA kit at 4℃
  • Product Description
    specifical
    Principle of the assay: ouse ACE ELISA Kit was based on standard sandwich enzyme-linked immune-sorbent assay technology. A monoclonal antibody from rat specific for ACE has been precoated onto 96-well plates. Standards(NSO, L35-Q1264) and test samples are added to the wells, a biotinylated detection polyclonal antibody from goat specific for ACE is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the mouse ACE amount of sample captured in plate. Background: Angiotensin-converting enzyme (ACE), an exopeptidase, is a circulating enzyme that participates in the body's renin-angiotensin system(RAS), which mediates extracellular volume (i.e. that of the blood plasma, lymph and interstitial fluid), and arterial vasoconstriction. It is secreted by pulmonary and renal endothelial cells and catalyzes the conversion of decapeptide angiotensin I to octapeptide angiotensin II.Using a DNA marker at the growth hormone gene locus, which they characterized as 'extremely polymorphic'and which showed no recombination with ACE, ACE was mapped to 17q22-q24, consistent with the in situ hybridization mapping to 17q23. ACE, or kininase II, is a dipeptidyl carboxypeptidase that plays an important role in blood pressure regulation and electrolyte balance by hydrolyzing angiotensin I into angiotensin II, a potent vasopressor, and aldosterone-stimulating peptide. The enzyme is also able to inactivate bradykinin, a potent vasodilator.
  • Mouse Angiotensin-converting enzyme, soluble form Protein information
  • Uniprot ID ACE_MOUSE
  • Uniprot AC P09470; P22967; Q6GTS2;
  • UniGene Mm.754;
  • GeneID 11421
  • KEGG mmu:11421;
  • Mouse Angiotensin-converting enzyme, soluble form Protein SEQUENCE
  • SEQUENCE 1312 AA; 150918 MW; 7DF9F5BE91762DFF CRC64;

    MGAASGQRGR WPLSPPLLML SLLVLLLQPS PAPALDPGLQ PGNFSPDEAG

    AQLFAESYNS SAEVVMFQST VASWAHDTNI TEENARRQEE AALVSQEFAE

    VWGKKAKELY ESIWQNFTDS KLRRIIGSIR TLGPANLPLA QRQQYNSLLS

    NMSRIYSTGK VCFPNKTATC WSLDPELTNI LASSRSYAKL LFAWEGWHDA

    VGIPLKPLYQ DFTAISNEAY RQDDFSDTGA FWRSWYESPS FEESLEHIYH

    QLEPLYLNLH AYVRRALHRR YGDKYVNLRG PIPAHLLGDM WAQSWENIYD

    MVVPFPDKPN LDVTSTMVQK GWNATHMFRV SEEFFTSLGL SPMPPEFWAE

    SMLEKPTDGR EVVCHASAWD FYNRKDFRIK QCTRVTMEQL ATVHHEMGHV

    QYYLQYKDLH VSLRRGANPG FHEAIGDVLA LSVSTPAHLH KIGLLDHVTN

    DIESDINYLL KMALEKIAFL PFGYLVDQWR WGVFSGRTPP SRYNFDWWYL

    RTKYQGICPP VARNETHFDA GAKFHIPNVT PYIRYFVSFV LQFQFHQALC

    KEAGHQGPLH QCDIYQSAQA GAKLKQVLQA GCSRPWQEVL KDLVGSDALD

    AKALLEYFQP VSQWLEEQNQ RNGEVLGWPE NQWRPPLPDN YPEGIDLETD

    EAKADRFVEE YDRTAQVLLN EYAEANWQYN TNITIEGSKI LLEKSTEVSN

    HTLKYGTRAK TFDVSNFQNS SIKRIIKKLQ NLDRAVLPPK ELEEYNQILL

    DMETTYSLSN ICYTNGTCMP LEPDLTNMMA TSRKYEELLW AWKSWRDKVG

    RAILPFFPKY VEFSNKIAKL NGYTDAGDSW RSLYESDNLE QDLEKLYQEL

    QPLYLNLHAY VRRSLHRHYG SEYINLDGPI PAHLLGNMWA QTWSNIYDLV

    APFPSAPNID ATEAMIKQGW TPRRIFKEAD NFFTSLGLLP VPPEFWNKSM

    LEKPTDGREV VCHPSAWDFY NGKDFRIKQC TSVNMEDLVI AHHEMGHIQY

    FMQYKDLPVT FREGANPGFH EAIGDIMALS VSTPKHLYSL NLLSTEGSGY

    EYDINFLMKM ALDKIAFIPF SYLIDQWRWR VFDGSITKEN YNQEWWSLRL

    KYQGLCPPVP RSQGDFDPGS KFHVPANVPY VRYFVSFIIQ FQFHEALCRA

    AGHTGPLHKC DIYQSKEAGK LLADAMKLGY SKPWPEAMKL ITGQPNMSAS

    AMMNYFKPLT EWLVTENRRH GETLGWPEYN WAPNTARAEG STAESNRVNF

    LGLYLEPQQA RVGQWVLLFL GVALLVATVG LAHRLYNIRN HHSLRRPHRG

    PQFGSEVELR HS

  • UCSC uc007lxw.2; mouse. [P09470-2];



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