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Mouse Col17a1 ELISA Kit

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  • Alternative name

    Mouse 180 kDa bullous pemphigoid antigen 2 ELISA Kit;Mouse bullous pemphigoid antigen 2 ELISA Kit;Mouse BP180 ELISA Kit;Mouse Bpag2 ELISA Kit;Mouse Bpag ELISA Kit;Mouse collagen, type XVII, alpha 1 ELISA Kit;Mouse collagen alpha-1(XVII) chain ELISA Kit;Mouse procollagen, type XVII, alpha 1 ELISA Kit;
  • Catalog
    E049062
  • species
    Mouse
  • GeneCol17a1
  • SpecificityThis assay has high sensitivity and excellent specificity for detection of COL17A1. No significant cross-reactivity or interference between ACTN4and analogues was observed. NOTE: Limited by current skills and knowledge, it is impossible for us to complete the cross-reactivity detection between ACTN4and all the analogues, therefore, cross reaction may still exist in some cases.
  • SamplesSerum, Plasma , tissue homogenates,Cell culture supernates,Other biological fluids.
  • Sensitivity0.1 ng/mL.
  • Intended UseMouse Col17a1 ELISA Kit allows for the in vitro quantitative determination of Col17a1 , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
  • StorageStore the whole ELISA kit at 4℃
  • Product Categories/FamilySignal Transduction
  • Product Description
    specifical    Intended Uses||This ACTN4ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Mouse COL17A1. This ELISA kit for research use only, not for therapeutic or diagnostic applications! Principle of the Assay: ACTN4 ELISA kit applies the competitive enzyme immunoassay technique utilizing a polyclonal anti-ACTN4 antibody and an ACTN4-HRP conjugate. The assay sample and buffer are incubated together with ACTN4-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the ACTN4 concentration since ACTN4 from samples and ACTN4-HRP conjugate compete for the anti-ACTN4 antibody binding site. Since the number of sites is limited, as more sites are occupied by ACTN4 from the sample, fewer sites are left to bind ACTN4-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The ACTN4 concentration in each sample is interpolated from this standard curve.
  • Mouse 120 kDa linear IgA disease antigen homolog Protein information
  • Uniprot ID COHA1_MOUSE
  • Uniprot AC Q07563; Q08AT3; Q3UXX1; Q99LK8;
  • UniGene Mm.1225;
  • GeneID 12821
  • KEGG mmu:12821;
  • Mouse 120 kDa linear IgA disease antigen homolog Protein SEQUENCE

  • SEQUENCE 1470 AA; 147975 MW; 7C38356D6ED68347 CRC64;

    MDVTKKSKRD GTEVTERIVT ETVTTRLTSL PPKGSTSNGY AKTGSLGGGS

    RLEKQSLTHG SSGYINSSGS IRGNASTSSY RRTHSPASTL PNSPGSTFER

    KAHMTRHGTY EGSSSGNSSP EYPRKELASS STRGRSQTRE SEIRVRLQSA

    SPSTRWTELD EVKRLLKGSR SASASPTRNT SNTLPIPKKG TVETKTVTAS

    SHSVSGTYDS AILDTNFPPH MWSSTLPAGS SLGTYQNNIT AQSTSLLNTN

    AYSTGSVFGV PNNMASCSPT LHPGLSSCSS VFGMQNNLAP SSSVLSHGTT

    TASTAYGAKK NVPQPPTVTS TGVSTSATCT TSVQSDDLLH KDCKFLILEK

    DNTPAKKEME LLIMTKDSGK VFTASPATIS STSFSEDTLK KEKQAAYAAD

    TCLKADVNGD LNTVSTKSKM TSAENHGYDR GGGGGRGKGG GAGGGGGGGG

    ASGGGGAWGA APAWCPCGSC CSWWKWLLGL LLTWLLLLGL LFGLIALAEE

    VRKLKARVEE LEKTKVLYHD VQMDKSNRDR LQAEAPSLGP GLGKAELDGY

    SQEAIWLFVR NKLMTEQENG NLRGSPGPKG DMGSQGPKGD RGLPGTPGIP

    GPLGHPGPEG PKGQKGSIGD PGMEGPIGQR GLAGPMGPRG EPGPPGSGEK

    GDRGIAGEQG PQGLPGVPGP PGLRGHSGSP GPQGPPGAVG PQGLRGDVGL

    PGVKGDKGLM GPPGPKGDQG EKGPRGLTGE PGIRGLPGAV GEPGAKGAMG

    PAGADGQQGS RGEQGLTGMP GTRGPPGPAG DPGKPGLTGP QGPQGLPGSP

    GRPGTKGEPG APGRVMTSEG SSTITVPGPP GPPGAMGPPG PPGTPGPAGP

    AGLPGQQGPR GEPGLAGDSF LSSGSSISEV LSAQGVDLRG PPGPPGPRGP

    PGPSIPGPPG PRGPPGEGVP GPPGPPGSFL TDSETFFTGP PGPPGPPGPK

    GDQGDPGVPG TPGISGGLSH GASSSTLYMQ GPPGPPGPPG PPGSLSSSGQ

    DIQHYIAEYM QSDNIRTYLS GVQGPPGPPG PPGPVITITG ETFDYSQLAS

    QVVSYLRSSG YGAGLSSASS SEDILAMLRR NDVWQYLRQN LVGPPGPPGP

    PGVSGDGSLL SLDYGELSRH ILNYMSSSGI SFGHPGPPGP PGLPGTSYEE

    LLTMLRGSDY RNIIGPPGPP GPPGMPGNAW SSISVEDLSS YLHTAGLSSI

    PGPPGPPGPP GPRGPPGVSA ALSTYAAENS DNFRSELISY LTSPDVRSFI

    VGPPGPPGPQ GPPGDGHLRE NYNWSSNSSA RRGTSYSSST GTGGTNGGSL

    GEGGAYGAGD GGPYGTDIGP GGGYGAAAGG GIYGTNGDSF RDGFTGDLDY

    NKLAVRVSES MQRQGLLQGM AYTVQGPPGP QGPPGISRVF SAYSNVTQDL

    MDFFQTYGTI PGPPGQKGDV GTPGPKGDRG PAGPRGPPGP PGPRGNKGEK

    GDKGDQVYTG RRKRSIAIKP

  • UCSC uc008hvj.2; mouse. [Q07563-1];



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