Mouse Cldn1 ELISA Kit

Alternative name
Mouse AI596271 ELISA Kit;Mouse claudin 1 ELISA Kit;Mouse claudin-1 ELISA Kit;
Catalog
E048585
species
Mouse
GeneCldn1
SpecificityThis assay has high sensitivity and excellent specificity for detection of CLDN-1. No significant cross-reactivity or interference between CLDN-1 and analogues was observed. NOTE: Limited by current skills and knowledge, it is impossible for us to complete the cross-reactivity detection between CLDN-1 and all the analogues, therefore, cross reaction may still exist in some cases.
SamplesSerum, Plasma , tissue homogenates,Cell culture supernates,Other biological fluids.
Sensitivity0.1 ng/mL.
Intended UseMouse Cldn1 ELISA Kit allows for the in vitro quantitative determination of Cldn1 , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
StorageStore the whole ELISA kit at 4℃
Product Categories/FamilySignal Transduction
Product Description
specificalIntended Uses: This CLDN-1 ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Mouse CLDN-1. This ELISA kit for research use only, not for therapeutic or diagnostic applications! Principle of the Assay||CLDN-1 ELISA kit applies the quantitative sandwich enzyme immunoassay technique. The microtiter plate has been pre-coated with a monoclonal antibody specific for CLDN-1. Standards or samples are then added to the microtiter plate wells and CLDN-1 if present, will bind to the antibody pre-coated wells. In order to quantitatively determine the amount of CLDN-1 present in the sample, a standardized preparation of horseradish peroxidase (HRP)-conjugated polyclonal antibody, specific for CLDN-1 are added to each well to "sandwich" the CLDN-1 immobilized on the plate. The microtiter plate undergoes incubation, and then the wells are thoroughly washed to remove all unbound components. Next, substrate solutions are added to each well. The enzyme (HRP) and substrate are allowed to react over a short incubation period. Only those wells that contain CLDN-1 and enzyme-conjugated antibody will exhibit a change in color. The enzyme-substrate reaction is terminated by addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The CLDN-1 concentration in each sample is interpolated from this standard curve.
Mouse Claudin-1 Protein information
Uniprot ID CLD1_MOUSE
Uniprot AC O88551;
UniGene Mm.289441;
GeneID 12737
KEGG mmu:12737;
Mouse Claudin-1 Protein SEQUENCE
SEQUENCE 211 AA; 22881 MW; BEF896FA62DBB6F0 CRC64;
MANAGLQLLG FILASLGWIG SIVSTALPQW KIYSYAGDNI VTAQAIYEGL
WMSCVSQSTG QIQCKVFDSL LNLNSTLQAT RALMVIGILL GLIAIFVSTI
GMKCMRCLED DEVQKMWMAV IGGIIFLISG LATLVATAWY GNRIVQEFYD
PLTPINARYE FGQALFTGWA AASLCLLGGV LLSCSCPRKT TSYPTPRPYP
KPTPSSGKDY V
UCSC uc007yuz.2; mouse.;

Category

Mouse Cldn1 ELISA Kit

Mouse AI596271 ELISA Kit;Mouse claudin 1 ELISA Kit;Mouse claudin-1 ELISA Kit;