Human B3GNT3 ELISA Kit

Alternative name
Human beta-1,3-galactosyl-O-glycosyl-glycoprotein beta-1,3-N-acetylglucosaminyltransferase ELISA Kit;Human Beta-1,3-galactosyltransferase 8 ELISA Kit;Human beta-1,3-GalTase 8 ELISA Kit;Human Beta3Gal-T8 ELISA Kit;Human beta3GalT8 ELISA Kit;Human B3GAL-T8 ELISA Kit;Human beta-3-Gx-T8 ELISA Kit;Human core 1 extending beta-1,3-N-acetylglucosaminyltransferase ELISA Kit;Human core1-beta3GlcNAcT ELISA Kit;Human transmembrane protein 3 ELISA Kit;Human UDP-Gal:beta-GlcNAc beta-1,3-galactosyltransferase 8 ELISA Kit;Human UDP-GlcNAc:betaGal beta-1,3-N-acetylglucosaminyltransferase 3 ELISA Kit;Human BGnT-3 ELISA Kit;Human beta-1,3-Gn-T3 ELISA Kit;Human Beta-1,3-N-acetylglucosaminyltransferase 3 ELISA Kit;Human beta3Gn-T3 ELISA Kit;Human UDP-galactose:beta-N-acetylglucosamine beta-1,3-galactosyltransferase 8 ELISA Kit;Human B3GALT8 ELISA Kit;Human TMEM3 ELISA Kit;Human B3GN-T3 ELISA Kit;Human B3GNT-3 ELISA Kit;Human HP10328 ELISA Kit;Human N-acetyllactosaminide beta-1,3-N-acetylglucosaminyltransferase 3 ELISA Kit;Human beta-1,3-N-acetylglucosaminyltransferase bGnT-3 ELISA Kit;Human putative type II membrane protein ELISA Kit;
Catalog
E004735
species
Human
GeneB3GNT3
Standard Curve
Other Species Mouse B3gnt3 ELISA Kit
SpecificityThis assay has high sensitivity and excellent specificity for detection of Beta-1,3-N-Acetylglucosaminyltransferase 3 (b3GNT3). No significant cross-reactivity or interference between Beta-1,3-N-Acetylglucosaminyltransferase 3 (b3GNT3) and analogues was observed.
SamplesSerum, Plasma , tissue homogenates,Cell culture supernates,Other biological fluids.
SensitivityTypically less than 0.066ng/mL.
Intended UseHuman B3GNT3 ELISA Kit allows for the in vitro quantitative determination of B3GNT3 , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
StorageStore the whole ELISA kit at 4℃
Product Description
specificalPrinciple of the Assay: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Beta-1,3-N-Acetylglucosaminyltransferase 3 (b3GNT3). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Beta-1,3-N-Acetylglucosaminyltransferase 3 (b3GNT3). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Beta-1,3-N-Acetylglucosaminyltransferase 3 (b3GNT3), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Beta-1,3-N-Acetylglucosaminyltransferase 3 (b3GNT3) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
Human N-acetyllactosaminide beta-1,3-N-acetylglucosaminyltransferase 3 Protein information
Uniprot ID B3GN3_HUMAN
Uniprot AC Q9Y2A9; B2RAS4; Q6NWU9; Q6NXU9; Q8WWR6; Q9C0J2;
UniGene Hs.657825; Hs.69009;
GeneID 10331
KEGG hsa:10331;
Human N-acetyllactosaminide beta-1,3-N-acetylglucosaminyltransferase 3 Protein SEQUENCE
SEQUENCE 372 AA; 42534 MW; A6E3FE88B2F00F10 CRC64;
MKYLRHRRPN ATLILAIGAF TLLLFSLLVS PPTCKVQEQP PAIPEALAWP
TPPTRPAPAP CHANTSMVTH PDFATQPQHV QNFLLYRHCR HFPLLQDVPP
SKCAQPVFLL LVIKSSPSNY VRRELLRRTW GRERKVRGLQ LRLLFLVGTA
SNPHEARKVN RLLELEAQTH GDILQWDFHD SFFNLTLKQV LFLQWQETRC
ANASFVLNGD DDVFAHTDNM VFYLQDHDPG RHLFVGQLIQ NVGPIRAFWS
KYYVPEVVTQ NERYPPYCGG GGFLLSRFTA AALRRAAHVL DIFPIDDVFL
GMCLELEGLK PASHSGIRTS GVRAPSQRLS SFDPCFYRDL LLVHRFLPYE
MLLMWDALNQ PNLTCGNQTQ IY
UCSC uc002nhl.2; human.;

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