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Alternative name
Allopregnanolone ELISA Kit,AP ELISA Kit,3α ELISA Kit,5α-tetrahydroprogesterone ELISA Kit,3α-hydroxy-5α-pregnan-20-one ELISA Kit
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Catalog
E001421
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species
Human
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GeneAP
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Other Species
Human AP/3a 5a THP ELISA KitHuman AP/3alpha-5alpha-THP ELISA KitHuman alpha2-AP ELISA KitHuman AP ELISA KitHuman AP-12 ELISA KitHuman AP-1 ELISA KitMouse AP/3alpha-5alpha-THP ELISA KitMouse alpha2-AP ELISA KitMouse AP ELISA KitMouse AP-1 ELISA KitBovine AP ELISA KitCanine AP ELISA KitChicken AP ELISA KitGeneral AP ELISA KitPorcine AP ELISA KitRabbit AP ELISA KitRat AP ELISA KitCamel AP ELISA KitGoat AP ELISA KitGuinea Pig AP ELISA KitHamster AP ELISA KitHorse AP ELISA KitMonkey AP ELISA KitSheep AP ELISA Kit
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SpecificityNo cross-reaction with other factors
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SamplesSerum, Plasma , tissue homogenates,Cell culture supernates,Other biological fluids.
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Sensitivity12pg/ml
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Intended UseHuman AP ELISA Kit allows for the in vitro quantitative determination of AP , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
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StorageStore the whole kit at 4℃
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Product Description
specificalHuman AP ELISA Kit is based on the competitive binding enzyme immunoassay technique. The microtiter plate provided in th is kit has been pre-coated with an antibody specific to AP, During the reaction,AP in the sample or standard competes with a fixed amount of biotin-labeled AP for sites on a pre-coated Monoclonal antibody specific to AP. Excess conjugate and unbound sample or standard are washed from the plate. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The concentration of AP in the samples is then determined by comparing the O.D. of the samples to the standard curve.
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