Human ALCAM ELISA Kit

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  • Alternative name

    Human activated leukocyte cell adhesion molecule ELISA Kit;Human MEMD ELISA Kit;Human CD166 ELISA Kit;Human CD166 antigen ELISA Kit;

  • Catalog
    E000465
  • species
    Human
  • GeneALCAM
  • Standard CurveHuman ALCAM ELISA Kit
  • Other Species Mouse Alcam ELISA KitBovine ALCAM ELISA KitCanine ALCAM ELISA KitChicken ALCAM ELISA KitRabbit ALCAM ELISA KitRat Alcam ELISA Kit
  • SpecificityThis assay has high sensitivity and excellent specificity for detection of Activated Leukocyte Cell Adhesion Molecule (ALCAM). No significant cross-reactivity or interference between Activated Leukocyte Cell Adhesion Molecule (ALCAM) and analogues was observed.
  • SamplesSerum, Plasma , tissue homogenates,Cell culture supernates,Other biological fluids.
  • SensitivityTypically less than 0.072ng/mL.
  • Intended UseHuman ALCAM ELISA Kit allows for the in vitro quantitative determination of ALCAM , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
  • StorageStore the whole ELISA kit at 4℃
  • Product Description
    specifical
    Principle of the Assay: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Activated Leukocyte Cell Adhesion Molecule (ALCAM). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Activated Leukocyte Cell Adhesion Molecule (ALCAM). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Activated Leukocyte Cell Adhesion Molecule (ALCAM), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Activated Leukocyte Cell Adhesion Molecule (ALCAM) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
  • Human CD166 antigen Protein information
  • Uniprot ID CD166_HUMAN
  • Uniprot AC Q13740; B2RNS3; B4DTU0; O60892; Q1HGM8; Q1HGM9; Q6PEY4; Q6ZS95;
  • UniGene Hs.591293; Hs.740441;
  • GeneID 214
  • KEGG hsa:214;
  • Human CD166 antigen Protein SEQUENCE
  • SEQUENCE 583 AA; 65102 MW; 68FEFD67C14B0C57 CRC64;

    MESKGASSCR LLFCLLISAT VFRPGLGWYT VNSAYGDTII IPCRLDVPQN

    LMFGKWKYEK PDGSPVFIAF RSSTKKSVQY DDVPEYKDRL NLSENYTLSI

    SNARISDEKR FVCMLVTEDN VFEAPTIVKV FKQPSKPEIV SKALFLETEQ

    LKKLGDCISE DSYPDGNITW YRNGKVLHPL EGAVVIIFKK EMDPVTQLYT

    MTSTLEYKTT KADIQMPFTC SVTYYGPSGQ KTIHSEQAVF DIYYPTEQVT

    IQVLPPKNAI KEGDNITLKC LGNGNPPPEE FLFYLPGQPE GIRSSNTYTL

    TDVRRNATGD YKCSLIDKKS MIASTAITVH YLDLSLNPSG EVTRQIGDAL

    PVSCTISASR NATVVWMKDN IRLRSSPSFS SLHYQDAGNY VCETALQEVE

    GLKKRESLTL IVEGKPQIKM TKKTDPSGLS KTIICHVEGF PKPAIQWTIT

    GSGSVINQTE ESPYINGRYY SKIIISPEEN VTLTCTAENQ LERTVNSLNV

    SAISIPEHDE ADEISDENRE KVNDQAKLIV GIVVGLLLAA LVAGVVYWLY

    MKKSKTASKH VNKDLGNMEE NKKLEENNHK TEA

  • UCSC uc003dvx.4; human. [Q13740-1];


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