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  • Alternative name

    Neutrophil elastase ELISA KIT; Bone marrow serine protease ELISA KIT; Elastase-2 ELISA KIT; Human leukocyte elastase ELISA KIT; HLE ELISA KIT; Medullasin ELISA KIT; PMN elastaseELANE ELISA KIT; ELA2 ELISA KIT; HLE ELISA KIT

  • Catalog
  • species
  • GeneANEA
  • Standard CurveHuman ANEA ELISA Kit
  • Other Species Mouse ANEA ELISA Kit
  • SamplesSerum, Plasma , tissue homogenates,Cell culture supernates,Other biological fluids.
  • Sensitivity1.0 mU/mL
  • Intended UseHuman ANEA ELISA Kit allows for the in vitro quantitative determination of ANEA , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
  • StorageFor 5-7days:Store the whole kit at 4℃
    For a Long time :Store the Substrate at 4℃, other reagent should store at -20℃.
  • Product Categories/FamilyNeurobiology
  • Product Description
    For Sample Serum, plasma, cell culture supernatants, body fluid and tissue homogenate INTENDED USE This NE-Ab ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Human NE-Ab. This ELISA kit for research use only, not for therapeutic or diagnostic applications! Principle of the Assay: NE-Ab ELISA kit applies the competitive enzyme immunoassay technique utilizing NE antigen and an NE-Ab-HRP conjugate. The assay sample and buffer are incubated together with NE-Ab-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the NE-Ab concentration since NE-Ab from samples and NE-Ab-HRP conjugate compete for the NE antigen binding site. Since the number of sites is limited, as more sites are occupied by NE-Ab from the sample, fewer sites are left to bind NE-Ab-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The NE-Ab concentration in each sample is interpolated from this standard curve.

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