Intended UseHuman P III NP ELISA Kit allows for the in vitro quantitative determination of P III NP , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
StorageFor 5-7days:Store the whole kit at 4℃
For a Long time :Store the Substrate at 4℃, other reagent should store at -20℃.
Product Categories/FamilyCell Biology
Product Description specificalPrinciple of the Assay: P3NP ELISA kit applies the quantitative sandwich enzyme immunoassay technique. The microtiter plate has been pre-coated with a monoclonal antibody specific for P3NP. Standards or samples are then added to the microtiter plate wells and P3NP if present, will bind to the antibody pre-coated wells. In order to quantitatively determine the amount of P3NP present in the sample, a standardized preparation of horseradish peroxidase (HRP) -conjugated polyclonal antibody, specific for P3NP are added to each well to "sandwich" the P3NP immobilized on the plate. The microtiter plate undergoes incubation, and then the wells are thoroughly washed to remove all unbound components. Next, substrate solutions are added to each well. The enzyme (HRP) and substrate are allowed to react over a short incubation period. Only those wells that contain P3NP and enzyme-conjugated antibody will exhibit a change in color. The enzyme-substrate reaction is terminated by addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The P3NP concentration in each sample is interpolated from this standard curve.