Human ADRB3 ELISA Kit

Alternative name
Human beta-3 adrenoreceptor ELISA Kit;Human beta-3 adrenoceptor ELISA Kit;Human ADRB3R ELISA Kit;Human B3AR ELISA Kit;Human BETA3AR ELISA Kit;Human adrenoceptor beta 3 ELISA Kit;Human beta-3 adrenergic receptor ELISA Kit;Human adrenergic, beta-3-, receptor ELISA Kit;
Catalog
E004649
species
Human
GeneADRB3
Standard Curve
Other Species Mouse Adrb3 ELISA Kit Bovine ADRB3 ELISA Kit Canine ADRB3 ELISA Kit Goat ADRB3 ELISA Kit Guinea Pig ADRB3 ELISA Kit Porcine ADRB3 ELISA Kit Rat Adrb3 ELISA Kit Sheep ADRB3 ELISA Kit
SpecificityThis assay has high sensitivity and excellent specificity for detection of ADRbeta3. No significant cross-reactivity or interference between ADRbeta3 and analogues was observed. NOTE: Limited by current skills and knowledge, it is impossible for us to complete the cross-reactivity detection between ADRbeta3 and all the analogues, therefore, cross reaction may still exist in some cases.
SamplesSerum, Plasma , tissue homogenates,Cell culture supernates,Other biological fluids.
Sensitivity1.0 pg/mL.
Intended UseHuman ADRB3 ELISA Kit allows for the in vitro quantitative determination of ADRB3 , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
StorageStore the whole ELISA kit at 4℃
Product Description
specificalPrinciple of the assay: ADRbeta3 ELISA kit applies the competitive enzyme immunoassay technique utilizing a monoclonal anti-ADRbeta3 antibody and an ADRbeta3-HRP conjugate. The assay sample and buffer are incubated together with ADRbeta3-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the ADRbeta3 concentration since ADRbeta3 from samples and ADRbeta3-HRP conjugate compete for the anti-ADRbeta3 antibody binding site. Since the number of sites is limited, as more sites are occupied by ADRbeta3 from the sample, fewer sites are left to bind ADRbeta3-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The ADRbeta3 concentration in each sample is interpolated from this standard curve.
Human Beta-3 adrenergic receptor Protein information
Uniprot ID ADRB3_HUMAN
Uniprot AC P13945; Q4JFT4;
UniGene Hs.2549;
GeneID 155
KEGG hsa:155;
Human Beta-3 adrenergic receptor Protein SEQUENCE
SEQUENCE 408 AA; 43519 MW; E98BD6C130DD977B CRC64;
MAPWPHENSS LAPWPDLPTL APNTANTSGL PGVPWEAALA GALLALAVLA
TVGGNLLVIV AIAWTPRLQT MTNVFVTSLA AADLVMGLLV VPPAATLALT
GHWPLGATGC ELWTSVDVLC VTASIETLCA LAVDRYLAVT NPLRYGALVT
KRCARTAVVL VWVVSAAVSF APIMSQWWRV GADAEAQRCH SNPRCCAFAS
NMPYVLLSSS VSFYLPLLVM LFVYARVFVV ATRQLRLLRG ELGRFPPEES
PPAPSRSLAP APVGTCAPPE GVPACGRRPA RLLPLREHRA LCTLGLIMGT
FTLCWLPFFL ANVLRALGGP SLVPGPAFLA LNWLGYANSA FNPLIYCRSP
DFRSAFRRLL CRCGRRLPPE PCAAARPALF PSGVPAARSS PAQPRLCQRL
DGASWGVS
UCSC uc003xkr.3; human.;

Category

Human ADRB3 ELISA Kit

Human beta-3 adrenoreceptor ELISA Kit;Human beta-3 adrenoceptor ELISA Kit;Human ADRB3R ELISA Kit;Human B3AR ELISA Kit;Human BETA3AR ELISA Kit;Human adrenoceptor beta 3 ELISA Kit;Human beta-3 adrenergic receptor ELISA Kit;Human adrenergic, beta-3-, receptor ELISA Kit;