-
Alternative name
Bone morphogenetic protein 2 ELISA KIT; Bone morphogenetic protein 2ABMP2 ELISA KIT; BMP2A ELISA KIT; BMP-2 ELISA KIT; BMP-2A ELISA KIT
-
Catalog
E045920
-
species
Mouse
-
GeneBMP-2
-
SamplesSerum, Plasma , tissue homogenates,Cell culture supernates,Other biological fluids.
-
Sensitivity< 2pg/ml
-
Intended UseMouse BMP-2 ELISA Kit allows for the in vitro quantitative determination of BMP-2 , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
-
StorageFor 5-7days:Store the whole kit at 4℃
For a Long time :Store the Substrate at 4℃, other reagent should store at -20℃.
-
Product Description
specificalPrinciple of the Assay: This kit was based on sandwich enzyme-linked immune-sorbent assay technology. Anti- BMP-2 polyclonal antibody was pre-coated onto 96-well plates. And the biotin conjugated anti- BMP-2 polyclonal antibody was used as detection antibodies. The standards, test samples and biotin conjugated detection antibody were added to the wells subsequently, and wash with wash buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with wash buffer. TMB substrates were used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the BMP-2 amount of sample captured in plate. Read the O.D. absorbance at 450nm in a microplate reader, and then the concentration of BMP-2 can be calculated.
Background: Bone morphogenetic protein 2 (BMP-2) belongs to the TGF-beta superfamily of proteins. Like other family members, it plays an important role in the development of bone and cartilage. It has been demonstrated to potently induce osteoblast differentiation in a variety of cell types.BMP-2 has an ability to induce the formation of ectopic cartilage when implanted subcutaneously.
-