Mouse Gadd45g ELISA Kit

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  • Alternative name

    Mouse Cytokine-responsive protein CR6 ELISA Kit;Mouse Cr6 ELISA Kit;

  • Catalog
    E054863
  • species
    Mouse
  • GeneGadd45g
  • SpecificityThis assay has high sensitivity and excellent specificity for detection of Growth Arrest And DNA Damage Inducible Protein Gamma (GADD45g). No significant cross-reactivity or interference between Growth Arrest And DNA Damage Inducible Protein Gamma (GADD45g) and analogues was observed.
  • SamplesSerum, Plasma , tissue homogenates,Cell culture supernates,Other biological fluids.
  • SensitivityTypically less than 0.225ng/mL.
  • Intended UseMouse Gadd45g ELISA Kit allows for the in vitro quantitative determination of Gadd45g , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
  • StorageStore the whole ELISA kit at 4℃
  • Product Description
    specifical
    Principle of the Assay: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Growth Arrest And DNA Damage Inducible Protein Gamma (GADD45g). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Growth Arrest And DNA Damage Inducible Protein Gamma (GADD45g). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Growth Arrest And DNA Damage Inducible Protein Gamma (GADD45g), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Growth Arrest And DNA Damage Inducible Protein Gamma (GADD45g) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
  • Mouse Growth arrest and DNA damage-inducible protein GADD45 gamma Protein information
  • Uniprot ID GA45G_MOUSE
  • Uniprot AC Q9Z111;
  • UniGene Mm.281298;
  • Mouse Growth arrest and DNA damage-inducible protein GADD45 gamma Protein SEQUENCE
  • SEQUENCE 159 AA; 17211 MW; 4B5996927C57988F CRC64;

    MTLEEVRGQD TVPESTARMQ GAGKALHELL LSAHGQGCLT AGVYESAKVL

    NVDPDNVTFC VLAADEEDEG DIALQIHFTL IQAFCCENDI DIVRVGDVQR

    LAAIVGADEE GGAPGDLHCI LISNPNEDTW KDPALEKLSL FCEESRSFND

    WVPSITLPE




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