Mouse Lfng ELISA Kit

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  • Alternative name

    Mouse O-fucosylpeptide 3-beta-N-acetylglucosaminyltransferase ELISA Kit;Mouse AW061165 ELISA Kit;Mouse LFNG O-fucosylpeptide 3-beta-N-acetylglucosaminyltransferase ELISA Kit;Mouse beta-1,3-N-acetylglucosaminyltransferase lunatic fringe ELISA Kit;Mouse lunatic fringe ELISA Kit;

  • Catalog
    E045625
  • species
    Mouse
  • GeneLfng
  • Standard CurveMouse Lfng ELISA Kit
  • Other Species Human LFNG ELISA KitBovine LFNG ELISA KitChicken LFNG ELISA KitRat Lfng ELISA Kit
  • SpecificityThis assay has high sensitivity and excellent specificity for detection of LFNG. No significant cross-reactivity or interference between LFNG and analogues was observed. NOTE: Limited by current skills and knowledge, it is impossible for us to complete the cross-reactivity detection between LFNG and all the analogues, therefore, cross reaction may still exist in some cases.
  • SamplesSerum, Plasma , tissue homogenates,Cell culture supernates,Other biological fluids.
  • Sensitivity0.1 ng/mL.
  • Intended UseMouse Lfng ELISA Kit allows for the in vitro quantitative determination of Lfng , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
  • StorageStore the whole ELISA kit at 4℃
  • Product Categories/FamilyNeurobiology
  • Product Description
    specifical
    Intended Uses: This LFNG ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Mouse LFNG. This ELISA kit for research use only, not for therapeutic or diagnostic applications! Principle of the Assay||LFNG ELISA kit applies the competitive enzyme immunoassay technique utilizing a polyclonal anti-LFNG antibody and an LFNG-HRP conjugate. The assay sample and buffer are incubated together with LFNG-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the LFNG concentration since LFNG from samples and LFNG-HRP conjugate compete for the anti-LFNG antibody binding site. Since the number of sites is limited, as more sites are occupied by LFNG from the sample, fewer sites are left to bind LFNG-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The LFNG concentration in each sample is interpolated from this standard curve.
  • Mouse Beta-1,3-N-acetylglucosaminyltransferase lunatic fringe Protein information
  • Uniprot ID LFNG_MOUSE
  • Uniprot AC O09010; Q3U659; Q8K3F1; Q9DC10;
  • UniGene Mm.12834;
  • GeneID 16848
  • KEGG mmu:16848;
  • Mouse Beta-1,3-N-acetylglucosaminyltransferase lunatic fringe Protein SEQUENCE
  • SEQUENCE 378 AA; 41952 MW; FD0A02597BF9AFED CRC64;

    MLQRCGRRLL LALVGALLAC LLVLTADPPP TPMPAERGRR ALRSLAGSSG

    GAPASGSRAA VDPGVLTREV HSLSEYFSLL TRARRDADPP PGVASRQGDG

    HPRPPAEVLS PRDVFIAVKT TRKFHRARLD LLFETWISRH KEMTFIFTDG

    EDEALAKLTG NVVLTNCSSA HSRQALSCKM AVEYDRFIES GKKWFCHVDD

    DNYVNLRALL RLLASYPHTQ DVYIGKPSLD RPIQATERIS EHKVRPVHFW

    FATGGAGFCI SRGLALKMGP WASGGHFMST AERIRLPDDC TIGYIVEALL

    GVPLIRSGLF HSHLENLQQV PTTELHEQVT LSYGMFENKR NAVHIKGPFS

    VEADPSRFRS VHCHLYPDTP WCPRSAIF

  • UCSC uc009ahu.1; mouse.;



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