Human APOL1 ELISA Kit

Alternative name
Human Apolipoprotein L ELISA Kit;Human APO-L ELISA Kit;Human APOL ELISA Kit;Human Apolipoprotein L-I ELISA Kit;Human APOL-I ELISA Kit;Human FSGS4 ELISA Kit;Human apolipoprotein L1 ELISA Kit;Human apolipoprotein L 1 ELISA Kit;Human apolipoprotein L1-B3 ELISA Kit;
Catalog
E003361
species
Human
GeneAPOL1
Standard Curve
Other Species Mouse APOL1 ELISA Kit
SamplesSerum, Plasma , tissue homogenates,Cell culture supernates,Other biological fluids.
Sensitivity0.1 ng/mL.
Intended UseHuman APOL1 ELISA Kit allows for the in vitro quantitative determination of APOL1 , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
StorageStore the whole ELISA kit at 4℃
Product Categories/FamilyCardiovascular
Product Description
specificalIntended Uses: This ApoL1 ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Human ApoL1. This ELISA kit for research use only, not for therapeutic or diagnostic applications! Principle of the Assay: ApoL1 ELISA kit applies the quantitative sandwich enzyme immunoassay technique. The microtiter plate has been pre-coated with a monoclonal antibody specific for ApoL1. Standards or samples are then added to the microtiter plate wells and ApoL1 if present, will bind to the antibody pre-coated wells. In order to quantitatively determine the amount of ApoL1 present in the sample, a standardized preparation of horseradish peroxidase (HRP)-conjugated polyclonal antibody, specific for ApoL1 are added to each well to "sandwich" the ApoL1 immobilized on the plate. The microtiter plate undergoes incubation, and then the wells are thoroughly washed to remove all unbound components. Next, substrate solutions are added to each well. The enzyme (HRP) and substrate are allowed to react over a short incubation period. Only those wells that contain ApoL1 and enzyme-conjugated antibody will exhibit a change in color. The enzyme-substrate reaction is terminated by addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The ApoL1 concentration in each sample is interpolated from this standard curve.
Human Apolipoprotein L1 Protein information
Uniprot ID APOL1_HUMAN
Uniprot AC Q96PM4; Q9BQ03;
UniGene Hs.114309;
GeneID 8542
KEGG hsa:8542;
Human Apolipoprotein L1 Protein SEQUENCE
SEQUENCE 398 AA; 43974 MW; BD1A8F1D7C5A889F CRC64;
MEGAALLRVS VLCIWMSALF LGVGVRAEEA GARVQQNVPS GTDTGDPQSK
PLGDWAAGTM DPESSIFIED AIKYFKEKVS TQNLLLLLTD NEAWNGFVAA
AELPRNEADE LRKALDNLAR QMIMKDKNWH DKGQQYRNWF LKEFPRLKSE
LEDNIRRLRA LADGVQKVHK GTTIANVVSG SLSISSGILT LVGMGLAPFT
EGGSLVLLEP GMELGITAAL TGITSSTMDY GKKWWTQAQA HDLVIKSLDK
LKEVREFLGE NISNFLSLAG NTYQLTRGIG KDIRALRRAR ANLQSVPHAS
ASRPRVTEPI SAESGEQVER VNEPSILEMS RGVKLTDVAP VSFFLVLDVV
YLVYESKHLH EGAKSETAEE LKKVAQELEE KLNILNNNYK ILQADQEL
UCSC uc003ape.4; human. [O14791-1];

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Category

Human APOL1 ELISA Kit

Human Apolipoprotein L ELISA Kit;Human APO-L ELISA Kit;Human APOL ELISA Kit;Human Apolipoprotein L-I ELISA Kit;Human APOL-I ELISA Kit;Human FSGS4 ELISA Kit;Human apolipoprotein L1 ELISA Kit;Human apolipoprotein L 1 ELISA Kit;Human apolipoprotein L1-B3 ELISA Kit;