Human APOL1 ELISA Kit

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  • Alternative name

    Human Apolipoprotein L ELISA Kit;Human APO-L ELISA Kit;Human APOL ELISA Kit;Human Apolipoprotein L-I ELISA Kit;Human APOL-I ELISA Kit;Human FSGS4 ELISA Kit;Human apolipoprotein L1 ELISA Kit;Human apolipoprotein L 1 ELISA Kit;Human apolipoprotein L1-B3 ELISA Kit;

  • Catalog
    E003361
  • species
    Human
  • GeneAPOL1
  • Standard CurveHuman APOL1 ELISA Kit
  • Other Species Mouse APOL1 ELISA Kit
  • SamplesSerum, Plasma , tissue homogenates,Cell culture supernates,Other biological fluids.
  • Sensitivity0.1 ng/mL.
  • Intended UseHuman APOL1 ELISA Kit allows for the in vitro quantitative determination of APOL1 , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
  • StorageStore the whole ELISA kit at 4℃
  • Product Categories/FamilyCardiovascular
  • Product Description
    specifical
    Intended Uses: This ApoL1 ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Human ApoL1. This ELISA kit for research use only, not for therapeutic or diagnostic applications! Principle of the Assay: ApoL1 ELISA kit applies the quantitative sandwich enzyme immunoassay technique. The microtiter plate has been pre-coated with a monoclonal antibody specific for ApoL1. Standards or samples are then added to the microtiter plate wells and ApoL1 if present, will bind to the antibody pre-coated wells. In order to quantitatively determine the amount of ApoL1 present in the sample, a standardized preparation of horseradish peroxidase (HRP)-conjugated polyclonal antibody, specific for ApoL1 are added to each well to "sandwich" the ApoL1 immobilized on the plate. The microtiter plate undergoes incubation, and then the wells are thoroughly washed to remove all unbound components. Next, substrate solutions are added to each well. The enzyme (HRP) and substrate are allowed to react over a short incubation period. Only those wells that contain ApoL1 and enzyme-conjugated antibody will exhibit a change in color. The enzyme-substrate reaction is terminated by addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The ApoL1 concentration in each sample is interpolated from this standard curve.
  • Human Apolipoprotein L1 Protein information
  • Uniprot ID APOL1_HUMAN
  • Uniprot AC Q96PM4; Q9BQ03;
  • UniGene Hs.114309;
  • GeneID 8542
  • KEGG hsa:8542;
  • Human Apolipoprotein L1 Protein SEQUENCE
  • SEQUENCE 398 AA; 43974 MW; BD1A8F1D7C5A889F CRC64;

    MEGAALLRVS VLCIWMSALF LGVGVRAEEA GARVQQNVPS GTDTGDPQSK

    PLGDWAAGTM DPESSIFIED AIKYFKEKVS TQNLLLLLTD NEAWNGFVAA

    AELPRNEADE LRKALDNLAR QMIMKDKNWH DKGQQYRNWF LKEFPRLKSE

    LEDNIRRLRA LADGVQKVHK GTTIANVVSG SLSISSGILT LVGMGLAPFT

    EGGSLVLLEP GMELGITAAL TGITSSTMDY GKKWWTQAQA HDLVIKSLDK

    LKEVREFLGE NISNFLSLAG NTYQLTRGIG KDIRALRRAR ANLQSVPHAS

    ASRPRVTEPI SAESGEQVER VNEPSILEMS RGVKLTDVAP VSFFLVLDVV

    YLVYESKHLH EGAKSETAEE LKKVAQELEE KLNILNNNYK ILQADQEL

  • UCSC uc003ape.4; human. [O14791-1];


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