Mouse Bak1 ELISA Kit

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  • Alternative name

    Mouse apoptosis regulator BAK ELISA Kit;Mouse Bak ELISA Kit;Mouse N-BAK1 ELISA Kit;Mouse N-Bak ELISA Kit;Mouse BCL2-antagonist/killer 1 ELISA Kit;Mouse bcl-2 homologous antagonist/killer ELISA Kit;Mouse Bcl2 homologous antagonist/killer ELISA Kit;

  • Catalog
    E045457
  • species
    Mouse
  • GeneBak1
  • Other Species Human BAK1 ELISA Kit
  • SpecificityThis assay has high sensitivity and excellent specificity for detection of Mouse BAK1. No significant cross-reactivity or interference between Mouse BAK1 and analogues was observed. Note: Limited by current skills and knowledge, it is impossible for us to complete the cross- reactivity detection between Mouse BAK1 and all the analogues, therefore, cross reaction may still exist.
  • SamplesSerum, Plasma , tissue homogenates,Cell culture supernates,Other biological fluids.
  • Intended UseMouse Bak1 ELISA Kit allows for the in vitro quantitative determination of Bak1 , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
  • StorageStore the whole ELISA kit at 4℃
  • Product Description
    specifical
    Description: The kit is a sandwich enzyme immunoassay for in vitro quantitative measurement of BAK1 in Mouse serum, plasma and other biological fluids Principle of the Assay: This ELISA kit uses Sandwich-ELISA as the method. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Mouse BAK1. Standards or samples are added to the appropriate micro ELISA plate wells and bound by the specific antibody. Then a biotinylated detection antibody specific for Mouse BAK1 and Avidin-Horseradish Peroxidase (HRP) conjugate is added to each micro plate well successively and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Mouse BAK1, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm +/- 2 nm. The OD value is proportional to the concentration of Mouse BAK1. You can calculate the concentration of Mouse BAK1 in the samples by comparing the OD of the samples to the standard curve.
  • Mouse Bcl-2 homologous antagonist/killer Protein information
  • Uniprot ID BAK_MOUSE
  • Uniprot AC O08734; E9QM21; Q8C264;
  • UniGene Mm.2443;
  • GeneID 12018
  • KEGG mmu:12018;
  • Mouse Bcl-2 homologous antagonist/killer Protein SEQUENCE
  • SEQUENCE 209 AA; 23295 MW; 49972D3CA21F4218 CRC64;

    MASGQGPGPP KVGCDESPSP SEQQVAQDTE EVFRSYVFYL HQQEQETQGA

    AAPANPEMDN LPLEPNSILG QVGRQLALIG DDINRRYDTE FQNLLEQLQP

    TAGNAYELFT KIASSLFKSG ISWGRVVALL GFGYRLALYV YQRGLTGFLG

    QVTCFLADII LHHYIARWIA QRGGWVAALN FRRDPILTVM VIFGVVLLGQ

    FVVHRFFRS

  • UCSC uc008bff.1; mouse.;


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