Human BACE1 ELISA Kit

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  • Alternative name

    Human aspartyl protease 2 ELISA Kit;Human ASP2 ELISA Kit;Human asp 2 ELISA Kit;Human Beta-site amyloid precursor protein cleaving enzyme 1 ELISA Kit;Human beta-site APP cleaving enzyme 1 ELISA Kit;Human memapsin-2 ELISA Kit;Human membrane-associated aspartic protease 2 ELISA Kit;Human BACE ELISA Kit;Human KIAA1149 ELISA Kit;Human HSPC104 ELISA Kit;Human beta-secretase 1 ELISA Kit;Human APP beta-secretase ELISA Kit;Human beta-secretase 1 precursor variant 1 ELISA Kit;Human beta-site APP-cleaving enzyme ELISA Kit;Human beta-site amyloid beta A4 precursor protein-cleaving enzyme ELISA Kit;Human transmembrane aspartic proteinase Asp2 ELISA Kit;

  • Catalog
    E004854
  • species
    Human
  • GeneBACE1
  • Standard CurveHuman BACE1 ELISA Kit
  • Other Species Mouse Bace1 ELISA KitBovine BACE1 ELISA KitGuinea Pig BACE1 ELISA KitRat Bace1 ELISA Kit
  • SpecificityThis assay has high sensitivity and excellent specificity for detection of Human BACE1. No significant cross-reactivity or interference between Human BACE1 and analogues was observed.
  • SamplesSerum, Plasma , tissue homogenates,Cell culture supernates,Other biological fluids.
  • Sensitivity0.78pg/ml
  • Intended UseHuman BACE1 ELISA Kit allows for the in vitro quantitative determination of BACE1 , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
  • StorageStore the whole ELISA kit at 4℃
  • Product Description
    specifical
    Principle of the Assay: The microtiter plate provided in this kit has been pre-coated with an antibody specific to BACE1. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated polyclonal antibody preparation specific for BACE1 and Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB (3,3',5,5' tetramethyl-benzidine) substrate solution is added to each well. Only those wells that contain BACE1, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm +/- 2 nm. The concentration of BACE1 in the samples is then determined by comparing the O.D. of the samples to the standard curve.
  • Human Beta-secretase 1 Protein information
  • Uniprot ID BACE1_HUMAN
  • Uniprot AC Q9UJT5; Q9ULS1;
  • UniGene Hs.504003;
  • GeneID 23621
  • KEGG hsa:23621;
  • Human Beta-secretase 1 Protein SEQUENCE
  • SEQUENCE 501 AA; 55711 MW; 377CE4C838C09F05 CRC64;

    MAQALPWLLL WMGAGVLPAH GTQHGIRLPL RSGLGGAPLG LRLPRETDEE

    PEEPGRRGSF VEMVDNLRGK SGQGYYVEMT VGSPPQTLNI LVDTGSSNFA

    VGAAPHPFLH RYYQRQLSST YRDLRKGVYV PYTQGKWEGE LGTDLVSIPH

    GPNVTVRANI AAITESDKFF INGSNWEGIL GLAYAEIARP DDSLEPFFDS

    LVKQTHVPNL FSLQLCGAGF PLNQSEVLAS VGGSMIIGGI DHSLYTGSLW

    YTPIRREWYY EVIIVRVEIN GQDLKMDCKE YNYDKSIVDS GTTNLRLPKK

    VFEAAVKSIK AASSTEKFPD GFWLGEQLVC WQAGTTPWNI FPVISLYLMG

    EVTNQSFRIT ILPQQYLRPV EDVATSQDDC YKFAISQSST GTVMGAVIME

    GFYVVFDRAR KRIGFAVSAC HVHDEFRTAA VEGPFVTLDM EDCGYNIPQT

    DESTLMTIAY VMAAICALFM LPLCLMVCQW CCLRCLRQQH DDFADDISLL

    K

  • UCSC uc001pqw.4; human. [P56817-1];




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