Human ACTN3 ELISA Kit

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  • Alternative name

    Human Alpha-actinin skeletal muscle isoform 3 ELISA Kit;Human F-actin cross-linking protein ELISA Kit;Human actinin alpha 3 (gene/pseudogene) ELISA Kit;Human alpha-actinin-3 ELISA Kit;Human actinin, alpha 3 ELISA Kit;Human alpha-actinin skeletal muscle ELISA Kit;

  • Catalog
    E000452
  • species
    Human
  • GeneACTN3
  • Standard CurveHuman ACTN3 ELISA Kit
  • Other Species Mouse Actn3 ELISA KitBovine ACTN3 ELISA Kit
  • SpecificityThis assay has high sensitivity and excellent specificity for detection of ACTN3. No significant cross-reactivity or interference between ACTN3 and analogues was observed. NOTE: Limited by current skills and knowledge, it is impossible for us to complete the cross-reactivity detection between ACTN3 and all the analogues, therefore, cross reaction may still exist in some cases.
  • SamplesSerum, Plasma , tissue homogenates,Cell culture supernates,Other biological fluids.
  • Sensitivity0.1ng/mL.
  • Intended UseHuman ACTN3 ELISA Kit allows for the in vitro quantitative determination of ACTN3 , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
  • StorageStore the whole ELISA kit at 4℃
  • Product Categories/FamilySignal Transduction
  • Product Description
    specifical
    Intended Uses: This ACTN3 ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Human ACTN3. This ELISA kit for research use only, not for therapeutic or diagnostic applications! Principle of the Assay||ACTN3 ELISA kit applies the competitive enzyme immunoassay technique utilizing a polyclonal anti-ACTN3 antibody and an ACTN3-HRP conjugate. The assay sample and buffer are incubated together with ACTN3-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the ACTN3 concentration since ACTN3 from samples and ACTN3-HRP conjugate compete for the anti-ACTN3 antibody binding site. Since the number of sites is limited, as more sites are occupied by ACTN3 from the sample, fewer sites are left to bind ACTN3-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The ACTN3 concentration in each sample is interpolated from this standard curve.
  • Human Alpha-actinin-3 Protein information
  • Uniprot ID ACTN3_HUMAN
  • Uniprot AC Q08043; A6NP77; Q4KKV2;
  • UniGene Hs.654432; Hs.737862;
  • GeneID 89
  • KEGG hsa:89;
  • Human Alpha-actinin-3 Protein SEQUENCE
  • SEQUENCE 901 AA; 103241 MW; C49B7885E7E1EDA7 CRC64;

    MMMVMQPEGL GAGEGRFAGG GGGGEYMEQE EDWDRDLLLD PAWEKQQRKT

    FTAWCNSHLR KAGTQIENIE EDFRNGLKLM LLLEVISGER LPRPDKGKMR

    FHKIANVNKA LDFIASKGVK LVSIGAEEIV DGNLKMTLGM IWTIILRFAI

    QDISVEETSA KEGLLLWCQR KTAPYRNVNV QNFHTSWKDG LALCALIHRH

    RPDLIDYAKL RKDDPIGNLN TAFEVAEKYL DIPKMLDAED IVNTPKPDEK

    AIMTYVSCFY HAFAGAEQAE TAANRICKVL AVNQENEKLM EEYEKLASEL

    LEWIRRTVPW LENRVGEPSM SAMQRKLEDF RDYRRLHKPP RIQEKCQLEI

    NFNTLQTKLR LSHRPAFMPS EGKLVSDIAN AWRGLEQVEK GYEDWLLSEI

    RRLQRLQHLA EKFRQKASLH EAWTRGKEEM LSQRDYDSAL LQEVRALLRR

    HEAFESDLAA HQDRVEHIAA LAQELNELDY HEAASVNSRC QAICDQWDNL

    GTLTQKRRDA LERMEKLLET IDRLQLEFAR RAAPFNNWLD GAVEDLQDVW

    LVHSVEETQS LLTAHDQFKA TLPEADRERG AIMGIQGEIQ KICQTYGLRP

    CSTNPYITLS PQDINTKWDM VRKLVPSCDQ TLQEELARQQ VNERLRRQFA

    AQANAIGPWI QAKVEEVGRL AAGLAGSLEE QMAGLRQQEQ NIINYKTNID

    RLEGDHQLLQ ESLVFDNKHT VYSMEHIRVG WEQLLTSIAR TINEVENQVL

    TRDAKGLSQE QLNEFRASFN HFDRKQNGMM EPDDFRACLI SMGYDLGEVE

    FARIMTMVDP NAAGVVTFQA FIDFMTRETA ETDTTEQVVA SFKILAGDKN

    YITPEELRRE LPAKQAEYCI RRMVPYKGSG APAGALDYVA FSSALYGESD

    L

  • UCSC uc021qlz.4; human.;




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