Human ACTN3 ELISA Kit

Alternative name
Human Alpha-actinin skeletal muscle isoform 3 ELISA Kit;Human F-actin cross-linking protein ELISA Kit;Human actinin alpha 3 (gene/pseudogene) ELISA Kit;Human alpha-actinin-3 ELISA Kit;Human actinin, alpha 3 ELISA Kit;Human alpha-actinin skeletal muscle ELISA Kit;
Catalog
E000452
species
Human
GeneACTN3
Standard Curve
Other Species Mouse Actn3 ELISA Kit Bovine ACTN3 ELISA Kit
SpecificityThis assay has high sensitivity and excellent specificity for detection of ACTN3. No significant cross-reactivity or interference between ACTN3 and analogues was observed. NOTE: Limited by current skills and knowledge, it is impossible for us to complete the cross-reactivity detection between ACTN3 and all the analogues, therefore, cross reaction may still exist in some cases.
SamplesSerum, Plasma , tissue homogenates,Cell culture supernates,Other biological fluids.
Sensitivity0.1ng/mL.
Intended UseHuman ACTN3 ELISA Kit allows for the in vitro quantitative determination of ACTN3 , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
StorageStore the whole ELISA kit at 4℃
Product Categories/FamilySignal Transduction
Product Description
specificalIntended Uses: This ACTN3 ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Human ACTN3. This ELISA kit for research use only, not for therapeutic or diagnostic applications! Principle of the Assay||ACTN3 ELISA kit applies the competitive enzyme immunoassay technique utilizing a polyclonal anti-ACTN3 antibody and an ACTN3-HRP conjugate. The assay sample and buffer are incubated together with ACTN3-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the ACTN3 concentration since ACTN3 from samples and ACTN3-HRP conjugate compete for the anti-ACTN3 antibody binding site. Since the number of sites is limited, as more sites are occupied by ACTN3 from the sample, fewer sites are left to bind ACTN3-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The ACTN3 concentration in each sample is interpolated from this standard curve.
Human Alpha-actinin-3 Protein information
Uniprot ID ACTN3_HUMAN
Uniprot AC Q08043; A6NP77; Q4KKV2;
UniGene Hs.654432; Hs.737862;
GeneID 89
KEGG hsa:89;
Human Alpha-actinin-3 Protein SEQUENCE
SEQUENCE 901 AA; 103241 MW; C49B7885E7E1EDA7 CRC64;
MMMVMQPEGL GAGEGRFAGG GGGGEYMEQE EDWDRDLLLD PAWEKQQRKT
FTAWCNSHLR KAGTQIENIE EDFRNGLKLM LLLEVISGER LPRPDKGKMR
FHKIANVNKA LDFIASKGVK LVSIGAEEIV DGNLKMTLGM IWTIILRFAI
QDISVEETSA KEGLLLWCQR KTAPYRNVNV QNFHTSWKDG LALCALIHRH
RPDLIDYAKL RKDDPIGNLN TAFEVAEKYL DIPKMLDAED IVNTPKPDEK
AIMTYVSCFY HAFAGAEQAE TAANRICKVL AVNQENEKLM EEYEKLASEL
LEWIRRTVPW LENRVGEPSM SAMQRKLEDF RDYRRLHKPP RIQEKCQLEI
NFNTLQTKLR LSHRPAFMPS EGKLVSDIAN AWRGLEQVEK GYEDWLLSEI
RRLQRLQHLA EKFRQKASLH EAWTRGKEEM LSQRDYDSAL LQEVRALLRR
HEAFESDLAA HQDRVEHIAA LAQELNELDY HEAASVNSRC QAICDQWDNL
GTLTQKRRDA LERMEKLLET IDRLQLEFAR RAAPFNNWLD GAVEDLQDVW
LVHSVEETQS LLTAHDQFKA TLPEADRERG AIMGIQGEIQ KICQTYGLRP
CSTNPYITLS PQDINTKWDM VRKLVPSCDQ TLQEELARQQ VNERLRRQFA
AQANAIGPWI QAKVEEVGRL AAGLAGSLEE QMAGLRQQEQ NIINYKTNID
RLEGDHQLLQ ESLVFDNKHT VYSMEHIRVG WEQLLTSIAR TINEVENQVL
TRDAKGLSQE QLNEFRASFN HFDRKQNGMM EPDDFRACLI SMGYDLGEVE
FARIMTMVDP NAAGVVTFQA FIDFMTRETA ETDTTEQVVA SFKILAGDKN
YITPEELRRE LPAKQAEYCI RRMVPYKGSG APAGALDYVA FSSALYGESD
L
UCSC uc021qlz.4; human.;

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Human ACTN3 ELISA Kit

Human Alpha-actinin skeletal muscle isoform 3 ELISA Kit;Human F-actin cross-linking protein ELISA Kit;Human actinin alpha 3 (gene/pseudogene) ELISA Kit;Human alpha-actinin-3 ELISA Kit;Human actinin, alpha 3 ELISA Kit;Human alpha-actinin skeletal muscle ELISA Kit;