Human NPHS2 ELISA Kit

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  • Alternative name

    Human PDCN ELISA Kit;Human SRN1 ELISA Kit;Human NPHS2, podocin ELISA Kit;Human podocin ELISA Kit;Human nephrosis 2, idiopathic, steroid-resistant (podocin) ELISA Kit;

  • Catalog
    E025962
  • species
    Human
  • GeneNPHS2
  • Other Species Mouse Nphs2 ELISA KitRat Nphs2 ELISA Kit
  • SpecificityThis assay has high sensitivity and excellent specificity for detection of Human NPHS2. No significant cross-reactivity or interference between Human NPHS2 and analogues was observed.
  • SamplesSerum, Plasma , tissue homogenates,Cell culture supernates,Other biological fluids.
  • Sensitivity0.078 ng/ml
  • Intended UseHuman NPHS2 ELISA Kit allows for the in vitro quantitative determination of NPHS2 , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
  • StorageStore the whole ELISA kit at 4℃
  • Product Description
    specifical
    Introduction: Podocin is a protein which lines the podocytes and assists in maintaining the barrier at the glomerular basement membrane. Podocin is a new member of the stomatin family, which consists of hairpin-like integral membrane proteins with intracellular NH2- and COOH-termini. Mutations in the podocin gene NPHS2 can cause nephrotic syndrome, such as Focal Segmental Glomerulosclerosis (FSGS) or Minimal Change Disease (MCD). Principle of the Assay: The microtiter plate provided in this kit has been pre-coated with an antibody specific to podocin. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody preparation specific for podocin and Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB (3,3',5,5' tetramethyl-benzidine) substrate solution is added to each well. Only those wells that contain podocin, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a 3 sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm +/- 2 nm. The concentration of podocin in the samples is then determined by comparing the O.D. of the samples to the standard curve.
  • Human Podocin Protein information
  • Uniprot ID PODO_HUMAN
  • Uniprot AC Q9NP85; B1AM32; B1AM33; Q8N6Q5;
  • UniGene Hs.412710; Hs.658505;
  • GeneID 7827
  • KEGG hsa:7827;
  • Human Podocin Protein SEQUENCE
  • SEQUENCE 383 AA; 42201 MW; BBB57783C840F752 CRC64;

    MERRARSSSR ESRGRGGRTP HKENKRAKAE RSGGGRGRQE AGPEPSGSGR

    AGTPGEPRAP AATVVDVDEV RGSGEEGTEV VALLESERPE EGTKSSGLGA

    CEWLLVLISL LFIIMTFPFS IWFCVKVVQE YERVIIFRLG HLLPGRAKGP

    GLFFFLPCLD TYHKVDLRLQ TLEIPFHEIV TKDMFIMEID AICYYRMENA

    SLLLSSLAHV SKAVQFLVQT TMKRLLAHRS LTEILLERKS IAQDAKVALD

    SVTCIWGIKV ERIEIKDVRL PAGLQHSLAV EAEAQRQAKV RMIAAEAEKA

    ASESLRMAAE ILSGTPAAVQ LRYLHTLQSL STEKPSTVVL PLPFDLLNCL

    SSPSNRTQGS LPFPSPSKPV EPLNPKKKDS PML

  • UCSC uc001gmq.5; human. [Q9NP85-1];


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