Mouse Arrb1 ELISA Kit

Alternative name
Mouse Arrestin beta-1 ELISA Kit;Mouse 1200006I17Rik ELISA Kit;Mouse AW208571 ELISA Kit;Mouse G430100A01Rik ELISA Kit;Mouse arrestin, beta 1 ELISA Kit;Mouse beta-arrestin-1 ELISA Kit;Mouse beta-arrestin1 ELISA Kit;
Catalog
E044859
species
Mouse
GeneArrb1
SpecificityThis assay has high sensitivity and excellent specificity for detection of ARRb1. No significant cross-reactivity or interference between ARRb1 and analogues was observed. NOTE: Limited by current skills and knowledge, it is impossible for us to complete the cross-reactivity detection between ARRb1 and all the analogues, therefore, cross reaction may still exist in some cases.
SamplesSerum, Plasma , tissue homogenates,Cell culture supernates,Other biological fluids.
Sensitivity0.1 ng/mL.
Intended UseMouse Arrb1 ELISA Kit allows for the in vitro quantitative determination of Arrb1 , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
StorageStore the whole ELISA kit at 4℃
Product Categories/FamilySignal Transduction
Product Description
specificalPrinciple of the assay: ARRb1 ELISA kit applies the competitive enzyme immunoassay technique utilizing a monoclonal anti-ARRb1 antibody and an ARRb1-HRP conjugate. The assay sample and buffer are incubated together with ARRb1-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the ARRb1 concentration since ARRb1 from samples and ARRb1-HRP conjugate compete for the anti-ARRb1 antibody binding site. Since the number of sites is limited, as more sites are occupied by ARRb1 from the sample, fewer sites are left to bind ARRb1-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The ARRb1 concentration in each sample is interpolated from this standard curve.
Mouse Beta-arrestin-1 Protein information
Uniprot ID ARRB1_MOUSE
Uniprot AC Q8BWG8; Q3UH95; Q8BTJ5;
UniGene Mm.260193;
GeneID 109689
KEGG mmu:109689;
Mouse Beta-arrestin-1 Protein SEQUENCE
SEQUENCE 418 AA; 46973 MW; 389C24F8CDA06E64 CRC64;
MGDKGTRVFK KASPNGKLTV YLGKRDFVDH IDLVDPVDGV VLVDPEYLKE
RRVYVTLTCA FRYGREDLDV LGLTFRKDLF VANVQSFPPA PEDKKPLTRL
QERLIKKLGE HACPFTFEIP PNLPCSVTLQ PGPEDTGKAC GVDYEVKAFC
AENLEEKIHK RNSVRLVIRK VQYAPERPGP QPTAETTRQF LMSDKPLHLE
ASLDKEIYYH GEPISVNVHV TNNTNKTVKK IKISVRQYAD ICLFNTAQYK
CPVAMEEADD NVAPSSTFCK VYTLTPFLAN NREKRGLALD GKLKHEDTNL
ASSTLLREGA NREILGIIVS YKVKVKLVVS RGGLLGDLAS SDVAVELPFT
LMHPKPKEEP PHREVPESET PVDTNLIELD TNDDDIVFED FARQRLKGMK
DDKDEEDDGT GSPHLNNR
UCSC uc009ilv.1; mouse. [Q8BWG8-2];

Category

Mouse Arrb1 ELISA Kit

Mouse Arrestin beta-1 ELISA Kit;Mouse 1200006I17Rik ELISA Kit;Mouse AW208571 ELISA Kit;Mouse G430100A01Rik ELISA Kit;Mouse arrestin, beta 1 ELISA Kit;Mouse beta-arrestin-1 ELISA Kit;Mouse beta-arrestin1 ELISA Kit;