Human IRAK1 ELISA Kit

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  • Alternative name

    Human IRAK-1 ELISA Kit;Human IRAK ELISA Kit;Human pelle ELISA Kit;Human interleukin 1 receptor associated kinase 1 ELISA Kit;Human interleukin-1 receptor-associated kinase 1 ELISA Kit;Human Pelle homolog ELISA Kit;

  • Catalog
    E020447
  • species
    Human
  • GeneIRAK1
  • Standard CurveHuman IRAK1 ELISA Kit
  • Other Species Mouse Irak1 ELISA KitBovine IRAK1 ELISA Kit
  • SpecificityThis assay has high sensitivity and excellent specificity for detection of Interleukin 1 Receptor Associated Kinase 1 (IRAK1). No significant cross-reactivity or interference between Interleukin 1 Receptor Associated Kinase 1 (IRAK1) and analogues was observed.
  • SamplesSerum, Plasma , tissue homogenates,Cell culture supernates,Other biological fluids.
  • SensitivityTypically less than 0.116ng/mL.
  • Intended UseHuman IRAK1 ELISA Kit allows for the in vitro quantitative determination of IRAK1 , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
  • StorageStore the whole ELISA kit at 4℃
  • Product Description
    specifical
    Principle of the Assay: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Interleukin 1 Receptor Associated Kinase 1 (IRAK1). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Interleukin 1 Receptor Associated Kinase 1 (IRAK1). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Interleukin 1 Receptor Associated Kinase 1 (IRAK1), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Interleukin 1 Receptor Associated Kinase 1 (IRAK1) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
  • Human Interleukin-1 receptor-associated kinase 1 Protein information
  • Uniprot ID IRAK1_HUMAN
  • Uniprot AC P51617; D3DWW3; D3DWW4; Q7Z5V4; Q96C06; Q96RL2;
  • UniGene Hs.522819;
  • GeneID 3654
  • KEGG hsa:3654;
  • Human Interleukin-1 receptor-associated kinase 1 Protein SEQUENCE
  • SEQUENCE 712 AA; 76537 MW; A7ADED75D3A3981D CRC64;

    MAGGPGPGEP AAPGAQHFLY EVPPWVMCRF YKVMDALEPA DWCQFAALIV

    RDQTELRLCE RSGQRTASVL WPWINRNARV ADLVHILTHL QLLRARDIIT

    AWHPPAPLPS PGTTAPRPSS IPAPAEAEAW SPRKLPSSAS TFLSPAFPGS

    QTHSGPELGL VPSPASLWPP PPSPAPSSTK PGPESSVSLL QGARPFPFCW

    PLCEISRGTH NFSEELKIGE GGFGCVYRAV MRNTVYAVKR LKENADLEWT

    AVKQSFLTEV EQLSRFRHPN IVDFAGYCAQ NGFYCLVYGF LPNGSLEDRL

    HCQTQACPPL SWPQRLDILL GTARAIQFLH QDSPSLIHGD IKSSNVLLDE

    RLTPKLGDFG LARFSRFAGS SPSQSSMVAR TQTVRGTLAY LPEEYIKTGR

    LAVDTDTFSF GVVVLETLAG QRAVKTHGAR TKYLKDLVEE EAEEAGVALR

    STQSTLQAGL AADAWAAPIA MQIYKKHLDP RPGPCPPELG LGLGQLACCC

    LHRRAKRRPP MTQVYERLEK LQAVVAGVPG HSEAASCIPP SPQENSYVSS

    TGRAHSGAAP WQPLAAPSGA SAQAAEQLQR GPNQPVESDE SLGGLSAALR

    SWHLTPSCPL DPAPLREAGC PQGDTAGESS WGSGPGSRPT AVEGLALGSS

    ASSSSEPPQI IINPARQKMV QKLALYEDGA LDSLQLLSSS SLPGLGLEQD

    RQGPEESDEF QS

  • UCSC uc004fjr.2; human. [P51617-1];


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