Mouse ARG ELISA Kit

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  • Catalog
    E044800
  • species
    Mouse
  • GeneARG
  • Standard CurveMouse ARG ELISA Kit
  • Other Species Human ARG ELISA KitHuman ARG ELISA KitHuman L-Arg ELISA KitMouse L-Arg ELISA KitBovine ARG ELISA KitCanine ARG ELISA KitChicken ARG ELISA KitGeneral ARG ELISA KitPorcine ARG ELISA KitRabbit ARG ELISA KitRat ARG ELISA KitCamel ARG ELISA KitGoat ARG ELISA KitGuinea Pig ARG ELISA KitHamster ARG ELISA KitHorse ARG ELISA KitMonkey ARG ELISA KitSheep ARG ELISA Kit
  • SamplesSerum, Plasma , tissue homogenates,Cell culture supernates,Other biological fluids.
  • Sensitivity1.0umol/L
  • Intended UseMouse ARG ELISA Kit allows for the in vitro quantitative determination of ARG , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
  • StorageFor 5-7days:Store the whole kit at 4℃
    For a Long time :Store the Substrate at 4℃, other reagent should store at -20℃.
  • Product Description
    specifical
    Intended Uses: This ARG ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Mouse ARG. This ELISA kit for research use only, not for therapeutic or diagnostic applications! Principle of the Assay: ARG ELISA kit applies the competitive enzyme immunoassay technique utilizing a monoclonal anti-ARG antibody and an ARG-HRP conjugate. The assay sample and buffer are incubated together with ARG-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the ARG concentration since ARG from samples and ARG-HRP conjugate compete for the anti-ARG antibody binding site. Since the number of sites is limited, as more sites are occupied by ARG from the sample, fewer sites are left to bind ARG-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The ARG concentration in each sample is interpolated from this standard curve.



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