Human IL12B ELISA Kit

Alternative name
Human IL-12B ELISA Kit;Human cytotoxic lymphocyte maturation factor 40 kDa subunit ELISA Kit;Human CLMF p40 ELISA Kit;Human IL-12 subunit p40 ELISA Kit;Human NK cell stimulatory factor chain 2 ELISA Kit;Human NKSF2 ELISA Kit;Human CLMF ELISA Kit;Human CLMF2 ELISA Kit;Human IMD28 ELISA Kit;Human IMD29 ELISA Kit;Human NKSF ELISA Kit;Human interleukin 12B ELISA Kit;Human interleukin-12 subunit beta ELISA Kit;Human IL12, subunit p40 ELISA Kit;Human interleukin 12, p40 ELISA Kit;Human interleukin 12B (natural killer cell stimulatory factor 2, cytotoxic lymphocyte maturation factor 2, p40) ELISA Kit;Human interleukin-12 beta chain ELISA Kit;Human natural killer cell stimulatory factor, 40 kD subunit ELISA Kit;
Catalog
E019343
species
Human
GeneIL12B
SpecificityThe Human IL-12 p40 ELISA Kit allows for the detection and quantification of endogenous levels of natural and/or recombinant Human IL-12 p40 proteins within the range of 62.5 pg/ml - 4000 pg/ml.
SamplesSerum, Plasma , tissue homogenates,Cell culture supernates,Other biological fluids.
Sensitivity30 pg/ml.
Intended UseHuman IL12B ELISA Kit allows for the in vitro quantitative determination of IL12B , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
StorageStore the whole ELISA kit at 4℃
Product Description
specificalPrinciple of the Assay||The Human IL-12 p40 ELISA (Enzyme-Linked Immunosorbent Assay) kit is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of Human IL-12 p40 in Cell Culture Supernatants, Serum, Plasma, Tissue Homogenates. This assay employs an antibody specific for Human IL-12 p40 coated on a 96-well plate. Standards and samples are pipetted into the wells and IL-12 p40 present in a sample is bound to the wells by the immobilized antibody. The wells are washed and biotinylated anti-Human IL-12 p40 antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of IL-12 p40 bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm. Background/Introduction: Interleukin-12 (IL12; formerly NKSF, for natural killer cell stimulatory factor, or CLMF, for cytotoxic lymphocyte maturation factor) is a novel cytokine cloned from B-cell lines. IL-12 is a heterodimeric molecule composed of p35 and p40 subunits. The larger 40 kDa subunit (p40) is a member of the cytokine receptor family, and the smaller 35 kDa subunit (p35) is related to IL6 and GCSF. Both IL-12 p40 (-/-) and p35 (-/-) mice fail to produce IL-12 p70 heterodimer. Interleukin (IL)-12 has been cloned on the basis of its ability to activate natural killer (NK) cells and promote the development of cytolytic T cells. With further understanding of its activities, IL-12 has emerged as an important cytokine, affecting both immune and hematologic functions. It has been shown to be necessary for the T cell independent induction of interferon (IFN)-gamma, critical for the initial suppression of bacterial and parasitic infection; for the development of a Th1 response, critical for effective host defense against intracellular pathogens; and for the activation of differentiated T lymphocytes of both CD4+ and CD8+ phenotype. The subunits map to different chromosomes: p40 (IL12B) to 5q31-q33 and p35 (IL12A) to 3p12-3q13.2.
Human Interleukin-12 subunit beta Protein information
Uniprot ID IL12B_HUMAN
Uniprot AC P29460;
UniGene Hs.674;
GeneID 3593
KEGG hsa:3593;
Human Interleukin-12 subunit beta Protein SEQUENCE
SEQUENCE 328 AA; 37169 MW; 118FA801B8F5BB2F CRC64;
MCHQQLVISW FSLVFLASPL VAIWELKKDV YVVELDWYPD APGEMVVLTC
DTPEEDGITW TLDQSSEVLG SGKTLTIQVK EFGDAGQYTC HKGGEVLSHS
LLLLHKKEDG IWSTDILKDQ KEPKNKTFLR CEAKNYSGRF TCWWLTTIST
DLTFSVKSSR GSSDPQGVTC GAATLSAERV RGDNKEYEYS VECQEDSACP
AAEESLPIEV MVDAVHKLKY ENYTSSFFIR DIIKPDPPKN LQLKPLKNSR
QVEVSWEYPD TWSTPHSYFS LTFCVQVQGK SKREKKDRVF TDKTSATVIC
RKNASISVRA QDRYYSSSWS EWASVPCS
UCSC uc003lxr.2; human.;

Category

Human IL12B ELISA Kit