Human B7H6 ELISA Kit

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  • Alternative name

    Natural cytotoxicity triggering receptor 3 ligand 1 ELISA KIT; B7 homolog 6 ELISA KIT; B7-H6NCR3LG1 ELISA KIT; B7H6 ELISA KIT; B7-H6 ELISA KIT

  • Catalog
    E004394
  • species
    Human
  • GeneB7H6
  • Standard CurveHuman B7H6 ELISA Kit
  • SpecificityThis assay recognizes recombinant and natural human B7-H6. No significant cross-reactivity or interference was observed.
  • SamplesSerum, Plasma , tissue homogenates,Cell culture supernates,Other biological fluids.
  • Intended UseHuman B7H6 ELISA Kit allows for the in vitro quantitative determination of B7H6 , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
  • StorageFor 5-7days:Store the whole kit at 4℃
    For a Long time :Store the Substrate at 4℃, other reagent should store at -20℃.
  • Product Description
    specifical
    Intended Uses: This immunoassay kit allows for the in vitro quantitative determination of human B7 homolog 6, B7-H6 concentrations in serum, Plasma, tissue homogenates and Cell culture supernates and Other biological fluids. Principle of the Assay||The microtiter plate provided in this kit has been pre-coated with an antibody specific to B7-H6. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated polyclonal antibody preparation specific for B7-H6 and Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. Only those wells that contain B7-H6, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm +/- 2 nm. The concentration of B7-H6 in the samples is then determined by comparing the O.D. of the samples to the standard curve.




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