Human 12-LO ELISA Kit

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  • Alternative name

    Arachidonate 12-lipoxygenase, 12S-type ELISA KIT; Lipoxin synthase 12-LO (EC:3.3.2.-) ELISA KIT; Platelet-type lipoxygenase 12ALOX12 ELISA KIT; 12LO ELISA KIT; LOG12 ELISA KIT; 12S-LOX ELISA KIT; 12S-lipoxygenase ELISA KIT

  • Catalog
  • species
  • Gene12-LO
  • Other Species Mouse 12-LO ELISA Kit
  • SamplesSerum, Plasma , tissue homogenates,Cell culture supernates,Other biological fluids.
  • Sensitivity<0.188ng/ml
  • Intended UseHuman 12-LO ELISA Kit allows for the in vitro quantitative determination of 12-LO , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
  • StorageFor 5-7days:Store the whole kit at 4℃
    For a Long time :Store the Substrate at 4℃, other reagent should store at -20℃.
  • Product Description
    Principle of the Assay||This kit was based on sandwich enzyme-linked immune-sorbent assay technology. anti-Human 12-LO antibody was pre-coated onto 96-well plates. And the biotin conjugated anti-Human 12-LO antibody was used as detection antibodies. The standards, test samples and biotin conjugated detection antibody were added to the wells subsequently, and wash with wash buffer. HRP-Streptavidin was added and unbound conjugates were washed away with wash buffer. TMB substrates were used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the Human 12-LO amount of sample captured in plate. Read the O.D. absorbance at 450nm in a microplate reader, and then the concentration of Human 12-LO can be calculated.

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