Human BLK ELISA Kit

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  • Alternative name

    Human b lymphocyte kinase ELISA Kit;Human p55-Blk ELISA Kit;Human MODY11 ELISA Kit;Human BLK proto-oncogene, Src family tyrosine kinase ELISA Kit;Human tyrosine-protein kinase Blk ELISA Kit;Human B lymphoid tyrosine kinase ELISA Kit;Human BLK nonreceptor tyrosine kinase ELISA Kit;

  • Catalog
    E004385
  • species
    Human
  • GeneBLK
  • Standard CurveHuman BLK ELISA Kit
  • Other Species Mouse Blk ELISA Kit
  • SpecificityThis assay has high sensitivity and excellent specificity for detection of B-Lymphoid Tyrosine Kinase (BLK). No significant cross-reactivity or interference between B-Lymphoid Tyrosine Kinase (BLK) and analogues was observed.
  • SamplesSerum, Plasma , tissue homogenates,Cell culture supernates,Other biological fluids.
  • SensitivityTypically less than 0.071ng/mL.
  • Intended UseHuman BLK ELISA Kit allows for the in vitro quantitative determination of BLK , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
  • StorageStore the whole ELISA kit at 4℃
  • Product Description
    specifical
    Principle of the Assay: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to B-Lymphoid Tyrosine Kinase (BLK). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to B-Lymphoid Tyrosine Kinase (BLK). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain B-Lymphoid Tyrosine Kinase (BLK), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of B-Lymphoid Tyrosine Kinase (BLK) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
  • Human Tyrosine-protein kinase Blk Protein information
  • Uniprot ID BLK_HUMAN
  • Uniprot AC P51451; Q16291; Q96IN1;
  • UniGene Hs.146591;
  • GeneID 640
  • KEGG hsa:640;
  • Human Tyrosine-protein kinase Blk Protein SEQUENCE
  • SEQUENCE 505 AA; 57706 MW; B5F739BEF8389176 CRC64;

    MGLVSSKKPD KEKPIKEKDK GQWSPLKVSA QDKDAPPLPP LVVFNHLTPP

    PPDEHLDEDK HFVVALYDYT AMNDRDLQML KGEKLQVLKG TGDWWLARSL

    VTGREGYVPS NFVARVESLE MERWFFRSQG RKEAERQLLA PINKAGSFLI

    RESETNKGAF SLSVKDVTTQ GELIKHYKIR CLDEGGYYIS PRITFPSLQA

    LVQHYSKKGD GLCQRLTLPC VRPAPQNPWA QDEWEIPRQS LRLVRKLGSG

    QFGEVWMGYY KNNMKVAIKT LKEGTMSPEA FLGEANVMKA LQHERLVRLY

    AVVTKEPIYI VTEYMARGCL LDFLKTDEGS RLSLPRLIDM SAQIAEGMAY

    IERMNSIHRD LRAANILVSE ALCCKIADFG LARIIDSEYT AQEGAKFPIK

    WTAPEAIHFG VFTIKADVWS FGVLLMEVVT YGRVPYPGMS NPEVIRNLER

    GYRMPRPDTC PPELYRGVIA ECWRSRPEER PTFEFLQSVL EDFYTATERQ

    YELQP

  • UCSC uc003wty.4; human.;


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