Human RAD18 ELISA Kit

Alternative name
Human postreplication repair protein RAD18 ELISA Kit;Human hHR18 ELISA Kit;Human hRAD18 ELISA Kit;Human RING finger protein 73 ELISA Kit;Human RING-type E3 ubiquitin transferase RAD18 ELISA Kit;Human RNF73 ELISA Kit;Human RAD18, E3 ubiquitin protein ligase ELISA Kit;Human E3 ubiquitin-protein ligase RAD18 ELISA Kit;Human RAD18 homolog ELISA Kit;Human RAD18, S. cerevisiae, homolog ELISA Kit;Human RING-type E3 ubiquitin transferase RAD18 ELISA Kit;Human postreplication repair protein hRAD18p ELISA Kit;
Catalog
E032736
species
Human
GeneRAD18
Other Species Mouse Rad18 ELISA Kit
SpecificityThis assay has high sensitivity and excellent specificity for detection of Human RAD18. No significant cross-reactivity or interference between Human RAD18 and analogues was observed.
SamplesSerum, Plasma , tissue homogenates,Cell culture supernates,Other biological fluids.
Sensitivity4.7 pg/ml
Intended UseHuman RAD18 ELISA Kit allows for the in vitro quantitative determination of RAD18 , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
StorageStore the whole ELISA kit at 4℃
Product Description
specificalPrinciple of the Assay: This assay employs the quantitative sandwich enzyme immunoassay technique. Antibody specific for RAD18 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any RAD18 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for RAD18 is added to the wells. After washing, avidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of RAD18 bound in the initial step. The color development is stopped and the intensity of the color is measured.
Human E3 ubiquitin-protein ligase RAD18 Protein information
Uniprot ID RAD18_HUMAN
Uniprot AC Q9NS91; Q58F55; Q9NRT6;
UniGene Hs.375684;
GeneID 56852
KEGG hsa:56852;
Human E3 ubiquitin-protein ligase RAD18 Protein SEQUENCE
SEQUENCE 495 AA; 56223 MW; 744A053A50C65DD7 CRC64;
MDSLAESRWP PGLAVMKTID DLLRCGICFE YFNIAMIIPQ CSHNYCSLCI
RKFLSYKTQC PTCCVTVTEP DLKNNRILDE LVKSLNFARN HLLQFALESP
AKSPASSSSK NLAVKVYTPV ASRQSLKQGS RLMDNFLIRE MSGSTSELLI
KENKSKFSPQ KEASPAAKTK ETRSVEEIAP DPSEAKRPEP PSTSTLKQVT
KVDCPVCGVN IPESHINKHL DSCLSREEKK ESLRSSVHKR KPLPKTVYNL
LSDRDLKKKL KEHGLSIQGN KQQLIKRHQE FVHMYNAQCD ALHPKSAAEI
VREIENIEKT RMRLEASKLN ESVMVFTKDQ TEKEIDEIHS KYRKKHKSEF
QLLVDQARKG YKKIAGMSQK TVTITKEDES TEKLSSVCMG QEDNMTSVTN
HFSQSKLDSP EELEPDREED SSSCIDIQEV LSSSESDSCN SSSSDIIRDL
LEEEEAWEAS HKNDLQDTEI SPRQNRRTRA AESAEIEPRN KRNRN
UCSC uc003brd.4; human.;

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Human RAD18 ELISA Kit