Mouse Alad ELISA Kit

Alternative name
Mouse ALADH ELISA Kit;Mouse porphobilinogen synthase ELISA Kit;Mouse Lv ELISA Kit;Mouse aminolevulinate, delta-, dehydratase ELISA Kit;Mouse delta-aminolevulinic acid dehydratase ELISA Kit;
Catalog
E043619
species
Mouse
GeneAlad
SpecificityThis assay has high sensitivity and excellent specificity for detection of ALAD. No significant cross-reactivity or interference between ALAD and analogues was observed. NOTE: Limited by current skills and knowledge, it is impossible for us to complete the cross-reactivity detection between ALAD and all the analogues, therefore, cross reaction may still exist in some cases.
SamplesSerum, Plasma , tissue homogenates,Cell culture supernates,Other biological fluids.
Sensitivity0.1 ng/mL.
Intended UseMouse Alad ELISA Kit allows for the in vitro quantitative determination of Alad , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
StorageStore the whole ELISA kit at 4℃
Product Categories/FamilyCardiovascular
Product Description
specificalIntended Uses: This ALAD ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Mouse ALAD. This ELISA kit for research use only, not for therapeutic or diagnostic applications! Principle of the Assay||ALAD ELISA kit applies the competitive enzyme immunoassay technique utilizing a monoclonal anti-ALAD antibody and an ALAD-HRP conjugate. The assay sample and buffer are incubated together with ALAD-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the ALAD concentration since ALAD from samples and ALAD-HRP conjugate compete for the anti-ALAD antibody binding site. Since the number of sites is limited, as more sites are occupied by ALAD from the sample, fewer sites are left to bind ALAD-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The ALAD concentration in each sample is interpolated from this standard curve.
Mouse Delta-aminolevulinic acid dehydratase Protein information
Uniprot ID HEM2_MOUSE
Uniprot AC P10518; Q3THV6;
UniGene Mm.6988;
GeneID 17025
KEGG mmu:17025;
Mouse Delta-aminolevulinic acid dehydratase Protein SEQUENCE
SEQUENCE 330 AA; 36024 MW; 84052DC911C153EB CRC64;
MHHQSVLHSG YFHPLLRSWQ TAASTVSASN LIYPIFVTDV PDDVQPIASL
PGVARYGVNQ LEEMLRPLVE AGLRCVLIFG VPSRVPKDEQ GSAADSEDSP
TIEAVRLLRK TFPSLLVACD VCLCPYTSHG HCGLLSENGA FLAEESRQRL
AEVALAYAKA GCQVVAPSDM MDGRVEAIKA ALLKHGLGNR VSVMSYSAKF
ASCFYGPFRD AAQSSPAFGD RRCYQLPPGA RGLALRAVAR DIQEGADMLM
VKPGLPYLDM VREVKDKHPE LPLAVYQVSG EFAMLWHGAQ AGAFDLRTAV
LETMTAFRRA GADIIITYFA PQLLKWLKEE
UCSC uc008tez.2; mouse.;

Category

Mouse Alad ELISA Kit

Mouse ALADH ELISA Kit;Mouse porphobilinogen synthase ELISA Kit;Mouse Lv ELISA Kit;Mouse aminolevulinate, delta-, dehydratase ELISA Kit;Mouse delta-aminolevulinic acid dehydratase ELISA Kit;