Mouse Aldh1a3 ELISA Kit

Alternative name
Mouse Aldehyde dehydrogenase 6 ELISA Kit;Mouse retinaldehyde dehydrogenase 3 ELISA Kit;Mouse RALDH-3 ELISA Kit;Mouse RALDH3 ELISA Kit;Mouse ALDH6 ELISA Kit;Mouse V1 ELISA Kit;Mouse aldehyde dehydrogenase family 1, subfamily A3 ELISA Kit;Mouse aldehyde dehydrogenase family 1 member A3 ELISA Kit;Mouse aldehyde dehydrogenase-6 ELISA Kit;
Catalog
E043183
species
Mouse
GeneAldh1a3
SamplesSerum, Plasma , tissue homogenates,Cell culture supernates,Other biological fluids.
Intended UseMouse Aldh1a3 ELISA Kit allows for the in vitro quantitative determination of Aldh1a3 , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
StorageStore the whole ELISA kit at 4℃
Product Description
specificalIntended Uses: This ADP ELISA kit is intended Laboratory for research use only and is not for use in diagnostic or therapeutic procedures.The Stop Solution changes the color from blue to yellow and the intensity of the color is measured at 450 nm using a spectrophotometer. In order to measure the concentration of ADP in the sample, this ADP ELISA Kit includes a set of calibration standards. The calibration standards are assayed at the same time as the samples and allow the operator to produce a standard curve of Optical Density versus ADP concentration. The concentration of ADP in the samples is then determined by comparing the O.D. of the samples to the standard curve. Principle of the Assay: This ADP enzyme linked immunosorbent assay applies a technique called a quantitative sandwich immunoassay. The microtiter plate provided in this kit has been pre-coated with a monoclonal antibody specific for ADP. Standards or samples are then added to the microtiter plate wells and ADP if present, will bind to the antibody pre-coated wells. In order to quantitatively determine the amount of ADP present in the sample, a standardized preparation of horseradish peroxidase (HRP)-conjugated polyclonal antibody, specific for ADP are added to each well to "sandwich" the ADP immobilized on the plate. The microtiter plate undergoes incubation, and then the wells are thoroughly washed to remove all unbound components. Next, A and B substrate solution is added to each well. The enzyme (HRP) and substrate are allowed to react over a short incubation period.Only those wells that contain ADP and enzyme-conjugated antibody will exhibit a change in colour. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the colour change is measured spectrophotometrically at a wavelength of 450 nm.
Mouse Aldehyde dehydrogenase family 1 member A3 Protein information
Uniprot ID AL1A3_MOUSE
Uniprot AC Q9JHW9; Q9EQP7; Q9JI72;
UniGene Mm.140988;
GeneID 56847
KEGG mmu:56847;
Mouse Aldehyde dehydrogenase family 1 member A3 Protein SEQUENCE
SEQUENCE 512 AA; 56157 MW; 5BBC6DEE41E58CFE CRC64;
MATTNGAVEN GQPDGKPPAL PRPIRNLEVK FTKIFINNDW HESKSGRKFA
TYNPSTLEKI CEVEEGDKPD VDKAVEAAQA AFQRGSPWRR LDALSRGQLL
HQLADLVERD RAILATLETM DTGKPFLHAF FVDLEGCIKT FRYFAGWADK
IQGRTIPTDD NVVCFTRHEP IGVCGAITPW NFPLLMLAWK LAPALCCGNT
VVLKPAEQTP LTALYLASLI KEVGFPPGVV NIVPGFGPTV GAAISSHPQI
NKIAFTGSTE VGKLVREAAS RSNLKRVTLE LGGKNPCIVC ADADLDLAVE
CAHQGVFFNQ GQCCTAASRV FVEEQVYGEF VRRSVEFAKK RPVGDPFDAK
TEQGPQIDQK QFDKILELIE SGKKEGAKLE CGGSAMEDRG LFIKPTVFSD
VTDNMRIAKE EIFGPVQPIL KFKNLEEVIK RANSTDYGLT AAVFTKNLDK
ALKLAAALES GTVWINCYNA FYAQAPFGGF KMSGNGRELG EYALAEYTEV
KTVTIKLEEK NP
UCSC uc009hhi.2; mouse.;

Category

Mouse Aldh1a3 ELISA Kit