Mouse Hnf4a ELISA Kit

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  • Alternative name

    Mouse HNF-4-alpha ELISA Kit;Mouse nuclear receptor subfamily 2 group A member 1 ELISA Kit;Mouse transcription factor 14 ELISA Kit;Mouse TCF-14 ELISA Kit;Mouse transcription factor HNF-4 ELISA Kit;Mouse HNF-4 ELISA Kit;Mouse Hnf4 ELISA Kit;Mouse Nr2a1 ELISA Kit;Mouse Tcf14 ELISA Kit;Mouse Hnf4alpha ELISA Kit;Mouse MODY1 ELISA Kit;Mouse hepatic nuclear factor 4, alpha ELISA Kit;Mouse hepatocyte nuclear factor 4-alpha ELISA Kit;Mouse HNF4 alpha ELISA Kit;Mouse Nuclear receptor 2A1 ELISA Kit;

  • Catalog
    E055596
  • species
    Mouse
  • GeneHnf4a
  • Other Species Human HNF4A ELISA KitRat Hnf4a ELISA Kit
  • SamplesSerum, Plasma , tissue homogenates,Cell culture supernates,Other biological fluids.
  • Sensitivity<23.4 pg/mL
  • Intended UseMouse Hnf4a ELISA Kit allows for the in vitro quantitative determination of Hnf4a , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
  • StorageStore the whole ELISA kit at 4℃
  • Product Description
    specifical
    Principle of the Assay||This kit was based on sandwich enzyme-linked immune-sorbent assay technology. anti-Mouse HNF4a antibody was pre-coated onto 96-well plates. And the biotin conjugated anti-Mouse HNF4a antibody was used as detection antibodies. The standards, test samples and biotin conjugated detection antibody were added to the wells subsequently, and wash with wash buffer. HRP-Streptavidin was added and unbound conjugates were washed away with wash buffer. TMB substrates were used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the Mouse HNF4a amount of sample captured in plate. Read the O.D. absorbance at 450nm in a microplate reader, and then the concentration of Mouse HNF4a can be calculated.
  • Mouse Hepatocyte nuclear factor 4-alpha Protein information
  • Uniprot ID HNF4A_MOUSE
  • Uniprot AC P49698; A2A5I5; A2ICH0; Q3UNX3; Q8CFY1;
  • UniGene Mm.202383;
  • GeneID 15378
  • KEGG mmu:15378;
  • Mouse Hepatocyte nuclear factor 4-alpha Protein SEQUENCE
  • SEQUENCE 474 AA; 52684 MW; 10843D528EAE87EE CRC64;

    MRLSKTLAGM DMADYSAALD PAYTTLEFEN VQVLTMGNDT SPSEGANLNS

    SNSLGVSALC AICGDRATGK HYGASSCDGC KGFFRRSVRK NHMYSCRFSR

    QCVVDKDKRN QCRYCRLKKC FRAGMKKEAV QNERDRISTR RSSYEDSSLP

    SINALLQAEV LSQQITSPIS GINGDIRAKK IANITDVCES MKEQLLVLVE

    WAKYIPAFCE LLLDDQVALL RAHAGEHLLL GATKRSMVFK DVLLLGNDYI

    VPRHCPELAE MSRVSIRILD ELVLPFQELQ IDDNEYACLK AIIFFDPDAK

    GLSDPGKIKR LRSQVQVSLE DYINDRQYDS RGRFGELLLL LPTLQSITWQ

    MIEQIQFIKL FGMAKIDNLL QEMLLGGSAS DAPHTHHPLH PHLMQEHMGT

    NVIVANTMPS HLSNGQMCEW PRPRGQAATP ETPQPSPPSG SGSESYKLLP

    GAITTIVKPP SAIPQPTITK QEAI

  • UCSC uc012cit.1; mouse. [P49698-2];


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