Mouse Atf6 ELISA Kit

Alternative name
Mouse cAMP-dependent transcription factor ATF-6 alpha ELISA Kit;Mouse activating transcription factor 6 alpha ELISA Kit;Mouse ATF6-alpha ELISA Kit;Mouse 9130025P16Rik ELISA Kit;Mouse 9630036G24 ELISA Kit;Mouse AA789574 ELISA Kit;Mouse Atf6alpha ELISA Kit;Mouse ESTM49 ELISA Kit;Mouse activating transcription factor 6 ELISA Kit;Mouse cyclic AMP-dependent transcription factor ATF-6 alpha ELISA Kit;
Catalog
E042610
species
Mouse
GeneAtf6
Standard Curve
Other Species Human ATF6 ELISA Kit Rat Atf6 ELISA Kit
SpecificityThis assay has high sensitivity and excellent specificity for detection of ATF-6. No significant cross-reactivity or interference between ATF-6 and analogues was observed. NOTE: Limited by current skills and knowledge, it is impossible for us to complete the cross-reactivity detection between ATF-6 and all the analogues, therefore, cross reaction may still exist in some cases.
SamplesSerum, Plasma , tissue homogenates,Cell culture supernates,Other biological fluids.
Sensitivity0.1 ng/mL.
Intended UseMouse Atf6 ELISA Kit allows for the in vitro quantitative determination of Atf6 , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
StorageStore the whole ELISA kit at 4℃
Product Categories/FamilyEpigenetics and Nuclear Signaling
Product Description
specificalPrinciple of the assay: ATF-6 ELISA kit applies the competitive enzyme immunoassay technique utilizing a monoclonal anti-ATF-6 antibody and an ATF-6-HRP conjugate. The assay sample and buffer are incubated together with ATF-6-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the ATF-6 concentration since ATF-6 from samples and ATF-6-HRP conjugate compete for the anti-ATF-6 antibody binding site. Since the number of sites is limited, as more sites are occupied by ATF-6 from the sample, fewer sites are left to bind ATF-6-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The ATF-6 concentration in each sample is interpolated from this standard curve.
Mouse Processed cyclic AMP-dependent transcription factor ATF-6 alpha Protein information
Uniprot ID ATF6A_MOUSE
Uniprot AC F6VAN0; B2RU98; Q8BZ84;
UniGene Mm.377046;
GeneID 226641
KEGG mmu:226641;
Mouse Processed cyclic AMP-dependent transcription factor ATF-6 alpha Protein SEQUENCE
SEQUENCE 656 AA; 72694 MW; C3ADCF7DF2CB3EC9 CRC64;
MESPFSPVLP HGPDEDWEST LFAELGYFTD TDDVHFDAAH EAYENNFDHL
NFDLDLMPWE SDLWSPGSHF CSDMKAEPQP LSPASSSCSI SSPRSTDSCS
STQHVPEELD LLSSSQSPLS LYGDSCNSPS SVEPLKEEKP VTGPGNKTEH
GLTPKKKIQM SSKPSVQPKP LLLPAAPKTQ TNASVPAKAI IIQTLPALMP
LAKQQSIISI QPAPTKGQTV LLSQPTVVQL QSPAVLSSAQ PVLAVTGGAA
QLPNHVVNVL PAPVVSSPVN GKLSVTKPVL QSATRSMGSD IAVLRRQQRM
IKNRESACQS RKKKKEYMLG LEARLKAALS ENEQLKKENG SLKRQLDEVV
SENQRLKVPS PKRRAVCVMI VLAFIMLNYG PMSMLEQESR RVKPSVSPAN
QRRHLLEFSA KEVKDTSDGD NQKDSYSYDH SVSNDKALMV LSEEPLLYMP
PPPCQPLINT TESLRLNHEL RGWVHRHEVE RTKSRRMTNS QQKARILQGA
LEQGSNSQLM AVQYTETTSI SRNSGSELQV YYASPGSYQG FFDAIRRRGD
TFYVVSFRRD HLLLPATTHN KTTRPKMSIV LPAININDNV INGQDYEVMM
QIDCQVMDTR ILHIKSSSVP PYLRDHQRNQ TSTFFGSPPT TTETTHVVST
IPESLQ
UCSC uc007dmj.1; mouse.;

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Mouse Atf6 ELISA Kit