Mouse Il33 ELISA Kit

Alternative name
Mouse Il-33 ELISA Kit;Mouse 9230117N10Rik ELISA Kit;Mouse Il1f11 ELISA Kit;Mouse NF-HEV ELISA Kit;Mouse interleukin 33 ELISA Kit;Mouse interleukin-33 ELISA Kit;Mouse nuclear factor from high endothelial venules ELISA Kit;
Catalog
E056367
species
Mouse
GeneIl33
SpecificityNatural and recombinant mouse IL-33
SamplesSerum, Plasma , tissue homogenates,Cell culture supernates,Other biological fluids.
Sensitivity< 10pg/ml
Intended UseMouse Il33 ELISA Kit allows for the in vitro quantitative determination of Il33 , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
StorageStore the whole ELISA kit at 4℃
Product Description
specificalPrinciple of the assay: mouse IL-33 ELISA Kit was based on standard sandwich enzyme-linked immune-sorbent assay technology. A monoclonal antibody from rat specific for IL-33 has been precoated onto 96-well plates. Standards(E.coli, S109-I266) and test samples are added to the wells, a biotinylated detection polyclonal antibody from goat specific for IL-33 is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the mouse IL-33 amount of sample captured in plate. Background: Interleukin 33 (IL-33) is a cytokine belonging to the IL-1 superfamily. By genomic sequence analysis, the IL33 gene is mapped to chromosome 9p24.1 and its mouse homolog to a syntenic region on chromosome 19qC1. The induction of type 2 cytokines by IL-33 in vivo is believed to induce the severe pathological changes observed in mucosal organs following administration of IL-33. IL33, an alarmin released from necrotic cells, is necessary for potent CD8 + T cell (CTL) responses to replicating, prototypic RNA, and DNA viruses in mice. IL33 prevented the downregulation of CXCR2 and inhibition of chemotaxis induced by activation of TLR4, and found that IL33 reverses the TLR4-induced reduction of CXCR2 expression via the inhibition of expression of GRK2.
Mouse Interleukin-33(109-266) Protein information
Uniprot ID IL33_MOUSE
Uniprot AC Q8BVZ5; Q2YEJ4; Q3TQN0; Q99L46;
UniGene Mm.182359;
GeneID 77125
KEGG mmu:77125;
Mouse Interleukin-33(109-266) Protein SEQUENCE
SEQUENCE 266 AA; 29991 MW; E03C2C297EB43E23 CRC64;
MRPRMKYSNS KISPAKFSST AGEALVPPCK IRRSQQKTKE FCHVYCMRLR
SGLTIRKETS YFRKEPTKRY SLKSGTKHEE NFSAYPRDSR KRSLLGSIQA
FAASVDTLSI QGTSLLTQSP ASLSTYNDQS VSFVLENGCY VINVDDSGKD
QEQDQVLLRY YESPCPASQS GDGVDGKKLM VNMSPIKDTD IWLHANDKDY
SVELQRGDVS PPEQAFFVLH KKSSDFVSFE CKNLPGTYIG VKDNQLALVE
EKDESCNNIM FKLSKI
UCSC uc008hec.2; mouse.;

Category

Mouse Il33 ELISA Kit

Mouse Il-33 ELISA Kit;Mouse 9230117N10Rik ELISA Kit;Mouse Il1f11 ELISA Kit;Mouse NF-HEV ELISA Kit;Mouse interleukin 33 ELISA Kit;Mouse interleukin-33 ELISA Kit;Mouse nuclear factor from high endothelial venules ELISA Kit;