Contact Us
  • Catalog
  • species
  • Gene8ISOPG
  • Standard CurveHuman 8ISOPG ELISA Kit
  • SpecificityThis assay recognizes recombinant and natural Human 8-iso-Prostaglandin F2alpha. No significant cross-reactivity or interference was observed.
  • SamplesSerum, Plasma , tissue homogenates,Cell culture supernates,Other biological fluids.
  • SensitivityThe minimum detectable dose of Human 8-iso-Prostaglandin F2alpha is typically less than 7.80 pg/mL.The sensitivity of this assay, or Lower Limit of Detection (LLD) was defined as the lowest protein concentration that could be differentiated from zero.
  • Intended UseHuman 8ISOPG ELISA Kit allows for the in vitro quantitative determination of 8ISOPG , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
  • StorageFor 5-7days:Store the whole kit at 4℃
    For a Long time :Store the Substrate at 4℃, other reagent should store at -20℃.
  • Product Description
    Principle of the Assay: The ELISA is based on the competitive binding enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with an antibody specific to 8-iso-Prostaglandin F2alpha, During the reaction, 8-iso-Prostaglandin F2alpha in the sample or standard competes with a fixed amount of biotin-labeled 8-iso-Prostaglandin F2alpha for sites on a pre-coated Monoclonal antibody specific to 8-iso-Prostaglandin F2alpha. Excess conjugate and unbound sample or standard are washed from the plate. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm +/- 2 nm. The concentration of 8-iso-Prostaglandin F2alpha in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Inquiry About Human 8ISOPG ELISA Kit If you hope to order it or contact us directly, please contact us via We will get back to you in 12hrs,Thanks!