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Catalog
E042221
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species
Human
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Gene8ISOPG
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Standard Curve
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SpecificityThis assay recognizes recombinant and natural Human 8-iso-Prostaglandin F2alpha. No significant cross-reactivity or interference was observed.
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SamplesSerum, Plasma , tissue homogenates,Cell culture supernates,Other biological fluids.
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SensitivityThe minimum detectable dose of Human 8-iso-Prostaglandin F2alpha is typically less than 7.80 pg/mL.The sensitivity of this assay, or Lower Limit of Detection (LLD) was defined as the lowest protein concentration that could be differentiated from zero.
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Intended UseHuman 8ISOPG ELISA Kit allows for the in vitro quantitative determination of 8ISOPG , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
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StorageFor 5-7days:Store the whole kit at 4℃
For a Long time :Store the Substrate at 4℃, other reagent should store at -20℃.
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Product Description
specificalPrinciple of the Assay: The ELISA is based on the competitive binding enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with an antibody specific to 8-iso-Prostaglandin F2alpha, During the reaction, 8-iso-Prostaglandin F2alpha in the sample or standard competes with a fixed amount of biotin-labeled 8-iso-Prostaglandin F2alpha for sites on a pre-coated Monoclonal antibody specific to 8-iso-Prostaglandin F2alpha. Excess conjugate and unbound sample or standard are washed from the plate. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm +/- 2 nm. The concentration of 8-iso-Prostaglandin F2alpha in the samples is then determined by comparing the O.D. of the samples to the standard curve.
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