Human RPSA ELISA Kit

Alternative name
Human 37 kDa laminin receptor precursor ELISA Kit;Human 37LRP ELISA Kit;Human 37/67 kDa laminin receptor ELISA Kit;Human LRP/LR ELISA Kit;Human 67 kDa laminin receptor ELISA Kit;Human 67LR ELISA Kit;Human colon carcinoma laminin-binding protein ELISA Kit;Human Laminin receptor 1 ELISA Kit;Human lamR ELISA Kit;Human laminin-binding protein precursor p40 ELISA Kit;Human LBP/p40 ELISA Kit;Human multidrug resistance-associated protein MGr1-Ag ELISA Kit;Human NEM/1CHD4 ELISA Kit;Human LAMBR ELISA Kit;Human LAMR1 ELISA Kit;Human ICAS ELISA Kit;Human LBP ELISA Kit;Human LRP ELISA Kit;Human SA ELISA Kit;Human p40 ELISA Kit;Human ribosomal protein SA ELISA Kit;Human 40S ribosomal protein SA ELISA Kit;Human 37 kDa laminin receptor ELISA Kit;Human laminin receptor 1 (67kD, ribosomal protein SA) ELISA Kit;
Catalog
E042028
species
Human
GeneRPSA
SpecificityThis assay has high sensitivity and excellent specificity for detection of RPSA. No significant cross-reactivity or interference between RPSA and analogues was observed. NOTE: Limited by current skills and knowledge, it is impossible for us to complete the cross-reactivity detection between RPSA and all the analogues, therefore, cross reaction may still exist in some cases.
SamplesSerum, Plasma , tissue homogenates,Cell culture supernates,Other biological fluids.
Sensitivity1.0 pg/mL.
Intended UseHuman RPSA ELISA Kit allows for the in vitro quantitative determination of RPSA , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
StorageStore the whole ELISA kit at 4℃
Product Categories/FamilyEpigenetics and Nuclear Signaling
Product Description
specificalPriciple of the assay: RPSA ELISA kit applies the competitive enzyme immunoassay technique utilizing a monoclonal anti-RPSA antibody and an RPSA-HRP conjugate. The assay sample and buffer are incubated together with RPSA-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the RPSA concentration since RPSA from samples and RPSA-HRP conjugate compete for the anti-RPSA antibody binding site. Since the number of sites is limited, as more sites are occupied by RPSA from the sample, fewer sites are left to bind RPSA-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The RPSA concentration in each sample is interpolated from this standard curve.
Human 40S ribosomal protein SA Protein information
Uniprot ID RSSA_HUMAN
Uniprot AC Q86VC0;
UniGene Hs.449909;
GeneID 3921
KEGG hsa:3921;
Human 40S ribosomal protein SA Protein SEQUENCE
SEQUENCE 295 AA; 32854 MW; C68DDB16B759E79E CRC64;
MSGALDVLQM KEEDVLKFLA AGTHLGGTNL DFQMEQYIYK RKSDGIYIIN
LKRTWEKLLL AARAIVAIEN PADVSVISSR NTGQRAVLKF AAATGATPIA
GRFTPGTFTN QIQAAFREPR LLVVTDPRAD HQPLTEASYV NLPTIALCNT
DSPLRYVDIA IPCNNKGAHS VGLMWWMLAR EVLRMRGTIS REHPWEVMPD
LYFYRDPEEI EKEEQAAAEK AVTKEEFQGE WTAPAPEFTA TQPEVADWSE
GVQVPSVPIQ QFPTEDWSAQ PATEDWSAAP TAQATEWVGA TTDWS
UCSC uc003cjp.4; human.;

Category

Human RPSA ELISA Kit