Human GCK ELISA Kit

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  • Alternative name

    Human hexokinase type IV ELISA Kit;Human HK IV ELISA Kit;Human Hexokinase-4 ELISA Kit;Human HK4 ELISA Kit;Human Hexokinase-D ELISA Kit;Human FGQTL3 ELISA Kit;Human GK ELISA Kit;Human GLK ELISA Kit;Human HHF3 ELISA Kit;Human HKIV ELISA Kit;Human HXKP ELISA Kit;Human LGLK ELISA Kit;Human MODY2 ELISA Kit;Human glucokinase ELISA Kit;Human ATP:D-hexose 6-phosphotransferase ELISA Kit;Human glucokinase (hexokinase 4) ELISA Kit;Human hexokinase D, pancreatic isozyme ELISA Kit;

  • Catalog
    E016565
  • species
    Human
  • GeneGCK
  • Standard CurveHuman GCK ELISA Kit
  • Other Species Mouse Gck ELISA KitRat Gck ELISA Kit
  • SpecificityThis assay has high sensitivity and excellent specificity for detection of GCK. No significant cross-reactivity or interference between GCK and analogues was observed. NOTE: Limited by current skills and knowledge, it is impossible for us to complete the cross-reactivity detection between GCK and all the analogues, therefore, cross reaction may still exist in some cases.
  • SamplesSerum, Plasma , tissue homogenates,Cell culture supernates,Other biological fluids.
  • Sensitivity0.1 ng/mL.
  • Intended UseHuman GCK ELISA Kit allows for the in vitro quantitative determination of GCK , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
  • StorageStore the whole ELISA kit at 4℃
  • Product Description
    specifical
    Principle of the assay: GCK ELISA kit applies the quantitative sandwich enzyme immunoassay technique. The microtiter plate has been pre-coated with a monoclonal antibody specific for GCK. Standards or samples are then added to the microtiter plate wells and GCK if present, will bind to the antibody pre-coated wells. In order to quantitatively determine the amount of GCK present in the sample, a standardized preparation of horseradish peroxidase (HRP)-conjugated polyclonal antibody, specific for GCK are added to each well to "sandwich" the GCK immobilized on the plate. The microtiter plate undergoes incubation, and then the wells are thoroughly washed to remove all unbound components. Next, substrate solutions are added to each well. The enzyme (HRP) and substrate are allowed to react over a short incubation period. Only those wells that contain GCK and enzyme-conjugated antibody will exhibit a change in color. The enzyme-substrate reaction is terminated by addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The GCK concentration in each sample is interpolated from this standard curve.
  • Human Glucokinase Protein information
  • Uniprot ID HXK4_HUMAN
  • Uniprot AC P35557; A4D2J2; A4D2J3; Q05810;
  • UniGene Hs.1270;
  • GeneID 2645
  • KEGG hsa:2645;
  • Human Glucokinase Protein SEQUENCE
  • SEQUENCE 465 AA; 52191 MW; 094D4A2F78096724 CRC64;

    MLDDRARMEA AKKEKVEQIL AEFQLQEEDL KKVMRRMQKE MDRGLRLETH

    EEASVKMLPT YVRSTPEGSE VGDFLSLDLG GTNFRVMLVK VGEGEEGQWS

    VKTKHQMYSI PEDAMTGTAE MLFDYISECI SDFLDKHQMK HKKLPLGFTF

    SFPVRHEDID KGILLNWTKG FKASGAEGNN VVGLLRDAIK RRGDFEMDVV

    AMVNDTVATM ISCYYEDHQC EVGMIVGTGC NACYMEEMQN VELVEGDEGR

    MCVNTEWGAF GDSGELDEFL LEYDRLVDES SANPGQQLYE KLIGGKYMGE

    LVRLVLLRLV DENLLFHGEA SEQLRTRGAF ETRFVSQVES DTGDRKQIYN

    ILSTLGLRPS TTDCDIVRRA CESVSTRAAH MCSAGLAGVI NRMRESRSED

    VMRITVGVDG SVYKLHPSFK ERFHASVRRL TPSCEITFIE SEEGSGRGAA

    LVSAVACKKA CMLGQ

  • UCSC uc003tkj.2; human. [P35557-1];



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