Human RPS3A ELISA Kit

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  • Alternative name

    Human v-fos transformation effector protein ELISA Kit;Human fte-1 ELISA Kit;Human FTE1 ELISA Kit;Human MFTL ELISA Kit;Human S3A ELISA Kit;Human ribosomal protein S3A ELISA Kit;Human 40S ribosomal protein S3a ELISA Kit;Human v-fos transformation effector protein 1 ELISA Kit;

  • Catalog
    E042022
  • species
    Human
  • GeneRPS3A
  • Standard CurveHuman RPS3A ELISA Kit
  • Other Species Mouse Rps3a ELISA KitBovine RPS3A ELISA KitMonkey RPS3A ELISA KitRat Rps3a ELISA Kit
  • SpecificityThis assay has high sensitivity and excellent specificity for detection of RPS3A. No significant cross-reactivity or interference between RPS3A and analogues was observed. NOTE: Limited by current skills and knowledge, it is impossible for us to complete the cross-reactivity detection between RPS3A and all the analogues, therefore, cross reaction may still exist in some cases.
  • SamplesSerum, Plasma , tissue homogenates,Cell culture supernates,Other biological fluids.
  • Sensitivity0.1 ng/mL.
  • Intended UseHuman RPS3A ELISA Kit allows for the in vitro quantitative determination of RPS3A , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
  • StorageStore the whole ELISA kit at 4℃
  • Product Categories/FamilyEpigenetics and Nuclear Signaling
  • Product Description
    specifical
    Principle of the assay: RPS3A ELISA kit applies the competitive enzyme immunoassay technique utilizing a monoclonal anti-RPS3A antibody and an RPS3A-HRP conjugate. The assay sample and buffer are incubated together with RPS3A-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the RPS3A concentration since RPS3A from samples and RPS3A-HRP conjugate compete for the anti-RPS3A antibody binding site. Since the number of sites is limited, as more sites are occupied by RPS3A from the sample, fewer sites are left to bind RPS3A-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The RPS3A concentration in each sample is interpolated from this standard curve.
  • Human 40S ribosomal protein S3a Protein information
  • Uniprot ID RS3A_HUMAN
  • Uniprot AC P61247; B2R4D4; D3DP05; P33443; P49241;
  • UniGene Hs.356572;
  • GeneID 6189
  • KEGG hsa:6189;
  • Human 40S ribosomal protein S3a Protein SEQUENCE
  • SEQUENCE 264 AA; 29945 MW; 000037AE195F7A9D CRC64;

    MAVGKNKRLT KGGKKGAKKK VVDPFSKKDW YDVKAPAMFN IRNIGKTLVT

    RTQGTKIASD GLKGRVFEVS LADLQNDEVA FRKFKLITED VQGKNCLTNF

    HGMDLTRDKM CSMVKKWQTM IEAHVDVKTT DGYLLRLFCV GFTKKRNNQI

    RKTSYAQHQQ VRQIRKKMME IMTREVQTND LKEVVNKLIP DSIGKDIEKA

    CQSIYPLHDV FVRKVKMLKK PKFELGKLME LHGEGSSSGK ATGDETGAKV

    ERADGYEPPV QESV

  • UCSC uc003ilz.4; human.;


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