Human RPS21 ELISA Kit

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  • Alternative name

    Human HLDF ELISA Kit;Human S21 ELISA Kit;Human ribosomal protein S21 ELISA Kit;Human 40S ribosomal protein S21 ELISA Kit;Human 8.2 kDa differentiation factor ELISA Kit;Human human leukemia differentiation factor ELISA Kit;

  • Catalog
    E042011
  • species
    Human
  • GeneRPS21
  • Standard CurveHuman RPS21 ELISA Kit
  • Other Species Mouse Rps21 ELISA KitBovine RPS21 ELISA KitPorcine RPS21 ELISA KitRat Rps21 ELISA Kit
  • SpecificityThis assay has high sensitivity and excellent specificity for detection of RPS21. No significant cross-reactivity or interference between RPS21 and analogues was observed. NOTE: Limited by current skills and knowledge, it is impossible for us to complete the cross-reactivity detection between RPS21 and all the analogues, therefore, cross reaction may still exist in some cases.
  • SamplesSerum, Plasma , tissue homogenates,Cell culture supernates,Other biological fluids.
  • Sensitivity1.0 pg/mL.
  • Intended UseHuman RPS21 ELISA Kit allows for the in vitro quantitative determination of RPS21 , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
  • StorageStore the whole ELISA kit at 4℃
  • Product Categories/FamilyEpigenetics and Nuclear Signaling
  • Product Description
    specifical
    Principle of the Assay: RPS21 ELISA kit applies the competitive enzyme immunoassay technique utilizing a polyclonal anti-RPS21 antibody and an RPS21-HRP conjugate. The assay sample and buffer are incubated together with RPS21-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the RPS21 concentration since RPS21 from samples and RPS21-HRP conjugate compete for the anti-RPS21 antibody binding site. Since the number of sites is limited, as more sites are occupied by RPS21 from the sample, fewer sites are left to bind RPS21-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The RPS21 concentration in each sample is interpolated from this standard curve.
  • Human 40S ribosomal protein S21 Protein information
  • Uniprot ID RS21_HUMAN
  • Uniprot AC P63220; P35265;
  • UniGene Hs.190968;
  • GeneID 6227
  • KEGG hsa:6227;
  • Human 40S ribosomal protein S21 Protein SEQUENCE
  • SEQUENCE 83 AA; 9111 MW; 6794AC1292A06BD3 CRC64;

    MQNDAGEFVD LYVPRKCSAS NRIIGAKDHA SIQMNVAEVD KVTGRFNGQF

    KTYAICGAIR RMGESDDSIL RLAKADGIVS KNF



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