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Human ALPL ELISA Kit

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  • Alternative name

    Human AP-TNAP ELISA Kit;Human TNSALP ELISA Kit;Human alkaline phosphatase liver/bone/kidney isozyme ELISA Kit;Human APTNAP ELISA Kit;Human HOPS ELISA Kit;Human TNAP ELISA Kit;Human alkaline phosphatase, liver/bone/kidney ELISA Kit;Human alkaline phosphatase, tissue-nonspecific isozyme ELISA Kit;Human liver/bone/kidney-type alkaline phosphatase ELISA Kit;Human tissue-nonspecific ALP ELISA Kit;
  • Catalog
    E001387
  • species
    Human
  • GeneALPL
  • Standard CurveHuman ALPL ELISA Kit
  • Other Species Mouse Alpl ELISA KitBovine ALPL ELISA KitChicken ALPL ELISA KitRat Alpl ELISA Kit
  • SpecificityThis assay has high sensitivity and excellent specificity for detection of Alkaline Phosphatase, Liver/Bone/Kidney (ALPL). No significant cross-reactivity or interference between Alkaline Phosphatase, Liver/Bone/Kidney (ALPL) and analogues was observed.
  • SamplesSerum, Plasma , tissue homogenates,Cell culture supernates,Other biological fluids.
  • SensitivityTypically less than 31pg/mL.
  • Intended UseHuman ALPL ELISA Kit allows for the in vitro quantitative determination of ALPL , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
  • StorageStore the whole ELISA kit at 4℃
  • Product Description
    specifical    Principle of the Assay: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Alkaline Phosphatase, Liver/Bone/Kidney (ALPL). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Alkaline Phosphatase, Liver/Bone/Kidney (ALPL). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Alkaline Phosphatase, Liver/Bone/Kidney (ALPL), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Alkaline Phosphatase, Liver/Bone/Kidney (ALPL) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
  • Human Alkaline phosphatase, tissue-nonspecific isozyme Protein information
  • Uniprot ID PPBT_HUMAN
  • Uniprot AC Q5BKZ5; Q5VTG5; Q6NZI8; Q8WU32; Q9UBK0;
  • UniGene Hs.75431;
  • GeneID 249
  • KEGG hsa:249;
  • Human Alkaline phosphatase, tissue-nonspecific isozyme Protein SEQUENCE

  • SEQUENCE 524 AA; 57305 MW; 71B45F17F6211900 CRC64;

    MISPFLVLAI GTCLTNSLVP EKEKDPKYWR DQAQETLKYA LELQKLNTNV

    AKNVIMFLGD GMGVSTVTAA RILKGQLHHN PGEETRLEMD KFPFVALSKT

    YNTNAQVPDS AGTATAYLCG VKANEGTVGV SAATERSRCN TTQGNEVTSI

    LRWAKDAGKS VGIVTTTRVN HATPSAAYAH SADRDWYSDN EMPPEALSQG

    CKDIAYQLMH NIRDIDVIMG GGRKYMYPKN KTDVEYESDE KARGTRLDGL

    DLVDTWKSFK PRYKHSHFIW NRTELLTLDP HNVDYLLGLF EPGDMQYELN

    RNNVTDPSLS EMVVVAIQIL RKNPKGFFLL VEGGRIDHGH HEGKAKQALH

    EAVEMDRAIG QAGSLTSSED TLTVVTADHS HVFTFGGYTP RGNSIFGLAP

    MLSDTDKKPF TAILYGNGPG YKVVGGEREN VSMVDYAHNN YQAQSAVPLR

    HETHGGEDVA VFSKGPMAHL LHGVHEQNYV PHVMAYAACI GANLGHCAPA

    SSAGSLAAGP LLLALALYPL SVLF

  • UCSC uc001bet.4; human. [P05186-1];


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