Human OXCT1 ELISA Kit

Alternative name
Human 3-oxoacid CoA-transferase 1 ELISA Kit;Human somatic-type succinyl-CoA:3-oxoacid CoA-transferase ELISA Kit;Human SCOT-s ELISA Kit;Human OXCT ELISA Kit;Human SCOT ELISA Kit;Human succinyl-CoA:3-ketoacid coenzyme A transferase 1, mitochondrial ELISA Kit;Human 3-oxoacid CoA transferase ELISA Kit;Human succinyl CoA:3-oxoacid CoA transferase ELISA Kit;Human succinyl-CoA:3-ketoacid-CoA transferase ELISA Kit;
Catalog
E041941
species
Human
GeneOXCT1
SpecificityThis assay has high sensitivity and excellent specificity for detection of OXCT1. No significant cross-reactivity or interference between OXCT1 and analogues was observed.
SamplesSerum, Plasma , tissue homogenates,Cell culture supernates,Other biological fluids.
SensitivityThe minimum detectable dose of human OXCT1 is typically less than 0.124ng/mL. The sensitivity of this assay, or Lower Limit of Detection (LLD) was defined as the lowest protein concentration that could be differentiated from zero. It was determined by add
Intended UseHuman OXCT1 ELISA Kit allows for the in vitro quantitative determination of OXCT1 , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
StorageStore the whole ELISA kit at 4℃
Product Description
specificalPrinciple of the assay: The microtiter plate provided in this kit has been pre-coated with an antibody specific to OXCT1. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to OXCT1. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain OXCT1, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm +/- 10nm. The concentration of OXCT1 in the samples is then determined by comparing the O.D. of the samples to the standard curve.
Human Succinyl-CoA:3-ketoacid coenzyme A transferase 1, mitochondrial Protein information
Uniprot ID SCOT1_HUMAN
Uniprot AC P55809; B2R5V2; B7Z528;
UniGene Hs.278277;
GeneID 5019
KEGG hsa:5019;
Human Succinyl-CoA:3-ketoacid coenzyme A transferase 1, mitochondrial Protein SEQUENCE
SEQUENCE 520 AA; 56158 MW; 54DA790FB8EDA546 CRC64;
MAALKLLSSG LRLCASARGS GATWYKGCVC SFSTSAHRHT KFYTDPVEAV
KDIPDGATVL VGGFGLCGIP ENLIDALLKT GVKGLTAVSN NAGVDNFGLG
LLLRSKQIKR MVSSYVGENA EFERQYLSGE LEVELTPQGT LAERIRAGGA
GVPAFYTPTG YGTLVQEGGS PIKYNKDGSV AIASKPREVR EFNGQHFILE
EAITGDFALV KAWKADRAGN VIFRKSARNF NLPMCKAAET TVVEVEEIVD
IGAFAPEDIH IPQIYVHRLI KGEKYEKRIE RLSIRKEGDG EAKSAKPGDD
VRERIIKRAA LEFEDGMYAN LGIGIPLLAS NFISPNITVH LQSENGVLGL
GPYPRQHEAD ADLINAGKET VTILPGASFF SSDESFAMIR GGHVDLTMLG
AMQVSKYGDL ANWMIPGKMV KGMGGAMDLV SSAKTKVVVT MEHSAKGNAH
KIMEKCTLPL TGKQCVNRII TEKAVFDVDK KKGLTLIELW EGLTVDDVQK
STGCDFAVSP KLMPMQQIAN
UCSC uc003jmn.4; human. [P55809-1];

Category

Human OXCT1 ELISA Kit