Human LCK ELISA Kit

Alternative name
Human leukocyte C-terminal Src kinase ELISA Kit;Human LSK ELISA Kit;Human lymphocyte cell-specific protein-tyrosine kinase ELISA Kit;Human Protein YT16 ELISA Kit;Human Proto-oncogene Lck ELISA Kit;Human t cell-specific protein-tyrosine kinase ELISA Kit;Human p56-LCK ELISA Kit;Human IMD22 ELISA Kit;Human YT16 ELISA Kit;Human p56lck ELISA Kit;Human pp58lck ELISA Kit;Human LCK proto-oncogene, Src family tyrosine kinase ELISA Kit;Human tyrosine-protein kinase Lck ELISA Kit;Human T-lymphocyte specific protein tyrosine kinase p56lck ELISA Kit;Human p56(LSTRA) protein-tyrosine kinase ELISA Kit;Human proto-oncogene tyrosine-protein kinase LCK ELISA Kit;
Catalog
E040162
species
Human
GeneLCK
SpecificityThis assay has high sensitivity and excellent specificity for detection of LCK. No significant cross-reactivity or interference between LCK and analogues was observed. NOTE: Limited by current skills and knowledge, it is impossible for us to complete the cross-reactivity detection between LCK and all the analogues, therefore, cross reaction may still exist in some cases.
SamplesSerum, Plasma , tissue homogenates,Cell culture supernates,Other biological fluids.
Sensitivity1.0 ng/mL.
Intended UseHuman LCK ELISA Kit allows for the in vitro quantitative determination of LCK , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
StorageStore the whole ELISA kit at 4℃
Product Categories/FamilySignal Transduction
Product Description
specificalIntended Uses: This LCK ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Human LCK. This ELISA kit for research use only, not for therapeutic or diagnostic applications! Principle of the Assay||LCK ELISA kit applies the competitive enzyme immunoassay technique utilizing a Polyclonal anti-LCK antibody and an LCK-HRP conjugate. The assay sample and buffer are incubated together with LCK-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the LCK concentration since LCK from samples and LCK-HRP conjugate compete for the anti-LCK antibody binding site. Since the number of sites is limited, as more sites are occupied by LCK from the sample, fewer sites are left to bind LCK-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The LCK concentration in each sample is interpolated from this standard curve.
Human Tyrosine-protein kinase Lck Protein information
Uniprot ID LCK_HUMAN
Uniprot AC Q96DW4; Q9NYT8;
UniGene Hs.470627;
GeneID 3932
KEGG hsa:3932;
Human Tyrosine-protein kinase Lck Protein SEQUENCE
SEQUENCE 509 AA; 58001 MW; 44BFF0D43FFB420D CRC64;
MGCGCSSHPE DDWMENIDVC ENCHYPIVPL DGKGTLLIRN GSEVRDPLVT
YEGSNPPASP LQDNLVIALH SYEPSHDGDL GFEKGEQLRI LEQSGEWWKA
QSLTTGQEGF IPFNFVAKAN SLEPEPWFFK NLSRKDAERQ LLAPGNTHGS
FLIRESESTA GSFSLSVRDF DQNQGEVVKH YKIRNLDNGG FYISPRITFP
GLHELVRHYT NASDGLCTRL SRPCQTQKPQ KPWWEDEWEV PRETLKLVER
LGAGQFGEVW MGYYNGHTKV AVKSLKQGSM SPDAFLAEAN LMKQLQHQRL
VRLYAVVTQE PIYIITEYME NGSLVDFLKT PSGIKLTINK LLDMAAQIAE
GMAFIEERNY IHRDLRAANI LVSDTLSCKI ADFGLARLIE DNEYTAREGA
KFPIKWTAPE AINYGTFTIK SDVWSFGILL TEIVTHGRIP YPGMTNPEVI
QNLERGYRMV RPDNCPEELY QLMRLCWKER PEDRPTFDYL RSVLEDFFTA
TEGQYQPQP
UCSC uc001bux.3; human. [P06239-1];

Category

Human LCK ELISA Kit