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Alternative name
Tumor necrosis factor ELISA KIT; Cachectin ELISA KIT; TNF-alpha ELISA KIT; Tumor necrosis factor ligand superfamily member 2 ELISA KIT; TNF-aTNF ELISA KIT; TNFA ELISA KIT; TNFSF2 ELISA KIT; TNF-a ELISA KIT; NTF ELISA KIT; ICD1 ELISA KIT; ICD2 ELISA KIT
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Catalog
E039889
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species
Human
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GeneTNFa
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SpecificityThis assay has high sensitivity and excellent specificity for detection of High Sensitive Tumor Necrosis Factor Alpha (TNFa).
No significant cross-reactivity or interference between High Sensitive Tumor Necrosis Factor Alpha (TNFa) and analogues was observed.
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SamplesSerum, Plasma , tissue homogenates,Cell culture supernates,Other biological fluids.
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SensitivityTypically less than 0.55pg/mL.
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Intended UseHuman TNFa ELISA Kit allows for the in vitro quantitative determination of TNFa , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
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StorageFor 5-7days:Store the whole kit at 4℃
For a Long time :Store the Substrate at 4℃, other reagent should store at -20℃.
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Product Description
specificalPrinciple of the Assay: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to High Sensitive Tumor Necrosis Factor Alpha (TNFa). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to High Sensitive Tumor Necrosis Factor Alpha (TNFa). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain High Sensitive Tumor Necrosis Factor Alpha (TNFa), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of High Sensitive Tumor Necrosis Factor Alpha (TNFa) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
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