Human ACTB ELISA Kit

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  • Alternative name

    Human Beta-actin ELISA Kit;Human BRWS1 ELISA Kit;Human PS1TP5BP1 ELISA Kit;Human actin beta ELISA Kit;Human actin, cytoplasmic 1 ELISA Kit;Human PS1TP5-binding protein 1 ELISA Kit;Human beta cytoskeletal actin ELISA Kit;

  • Catalog
    E000386
  • species
    Human
  • GeneACTB
  • Standard CurveHuman ACTB ELISA Kit
  • Other Species Mouse Actb ELISA KitBovine ACTB ELISA KitCanine ACTB ELISA KitGuinea Pig ACTB ELISA KitChicken ACTB ELISA KitHamster ACTB ELISA KitHorse ACTB ELISA KitMonkey ACTB ELISA KitPorcine ACTB ELISA KitRabbit ACTB ELISA KitRat Actb ELISA KitSheep ACTB ELISA Kit
  • SamplesSerum, Plasma , tissue homogenates,Cell culture supernates,Other biological fluids.
  • Sensitivity0.1 ng/mL.
  • Intended UseHuman ACTB ELISA Kit allows for the in vitro quantitative determination of ACTB , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
  • StorageStore the whole ELISA kit at 4℃
  • Product Categories/FamilyCell Biology
  • Product Description
    specifical
    Principle of the Assay: ACTbeta ELISA kit applies the quantitative sandwich enzyme immunoassay technique. The microtiter plate has been pre-coated with a monoclonal antibody specific for ACTbeta. Standards or samples are then added to the microtiter plate wells and ACTbeta if present, will bind to the antibody pre-coated wells. In order to quantitatively determine the amount of ACTbeta present in the sample, a standardized preparation of horseradish peroxidase (HRP) -conjugated polyclonal antibody, specific for ACTbeta are added to each well to "sandwich" the ACTbeta immobilized on the plate. The microtiter plate undergoes incubation, and then the wells are thoroughly washed to remove all unbound components. Next, substrate solutions are added to each well. The enzyme (HRP) and substrate are allowed to react over a short incubation period. Only those wells that contain ACTbeta and enzyme-conjugated antibody will exhibit a change in color. The enzyme-substrate reaction is terminated by addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The ACTbeta concentration in each sample is interpolated from this standard curve.
  • Human Actin, cytoplasmic 1, N-terminally processed Protein information
  • Uniprot ID ACTB_HUMAN
  • Uniprot AC Q96HG5;
  • UniGene Hs.520640;
  • GeneID 60
  • KEGG hsa:60;
  • Human Actin, cytoplasmic 1, N-terminally processed Protein SEQUENCE
  • SEQUENCE 375 AA; 41737 MW; 6AFD05CA94E360E2 CRC64;

    MDDDIAALVV DNGSGMCKAG FAGDDAPRAV FPSIVGRPRH QGVMVGMGQK

    DSYVGDEAQS KRGILTLKYP IEHGIVTNWD DMEKIWHHTF YNELRVAPEE

    HPVLLTEAPL NPKANREKMT QIMFETFNTP AMYVAIQAVL SLYASGRTTG

    IVMDSGDGVT HTVPIYEGYA LPHAILRLDL AGRDLTDYLM KILTERGYSF

    TTTAEREIVR DIKEKLCYVA LDFEQEMATA ASSSSLEKSY ELPDGQVITI

    GNERFRCPEA LFQPSFLGME SCGIHETTFN SIMKCDVDIR KDLYANTVLS

    GGTTMYPGIA DRMQKEITAL APSTMKIKII APPERKYSVW IGGSILASLS

    TFQQMWISKQ EYDESGPSIV HRKCF





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