Human ACTB ELISA Kit

Alternative name
Human Beta-actin ELISA Kit;Human BRWS1 ELISA Kit;Human PS1TP5BP1 ELISA Kit;Human actin beta ELISA Kit;Human actin, cytoplasmic 1 ELISA Kit;Human PS1TP5-binding protein 1 ELISA Kit;Human beta cytoskeletal actin ELISA Kit;
Catalog
E000386
species
Human
GeneACTB
Standard Curve
Other Species Mouse Actb ELISA Kit Bovine ACTB ELISA Kit Canine ACTB ELISA Kit Guinea Pig ACTB ELISA Kit Chicken ACTB ELISA Kit Hamster ACTB ELISA Kit Horse ACTB ELISA Kit Monkey ACTB ELISA Kit Porcine ACTB ELISA Kit Rabbit ACTB ELISA Kit Rat Actb ELISA Kit Sheep ACTB ELISA Kit
SamplesSerum, Plasma , tissue homogenates,Cell culture supernates,Other biological fluids.
Sensitivity0.1 ng/mL.
Intended UseHuman ACTB ELISA Kit allows for the in vitro quantitative determination of ACTB , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
StorageStore the whole ELISA kit at 4℃
Product Categories/FamilyCell Biology
Product Description
specificalPrinciple of the Assay: ACTbeta ELISA kit applies the quantitative sandwich enzyme immunoassay technique. The microtiter plate has been pre-coated with a monoclonal antibody specific for ACTbeta. Standards or samples are then added to the microtiter plate wells and ACTbeta if present, will bind to the antibody pre-coated wells. In order to quantitatively determine the amount of ACTbeta present in the sample, a standardized preparation of horseradish peroxidase (HRP) -conjugated polyclonal antibody, specific for ACTbeta are added to each well to "sandwich" the ACTbeta immobilized on the plate. The microtiter plate undergoes incubation, and then the wells are thoroughly washed to remove all unbound components. Next, substrate solutions are added to each well. The enzyme (HRP) and substrate are allowed to react over a short incubation period. Only those wells that contain ACTbeta and enzyme-conjugated antibody will exhibit a change in color. The enzyme-substrate reaction is terminated by addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The ACTbeta concentration in each sample is interpolated from this standard curve.
Human Actin, cytoplasmic 1, N-terminally processed Protein information
Uniprot ID ACTB_HUMAN
Uniprot AC Q96HG5;
UniGene Hs.520640;
GeneID 60
KEGG hsa:60;
Human Actin, cytoplasmic 1, N-terminally processed Protein SEQUENCE
SEQUENCE 375 AA; 41737 MW; 6AFD05CA94E360E2 CRC64;
MDDDIAALVV DNGSGMCKAG FAGDDAPRAV FPSIVGRPRH QGVMVGMGQK
DSYVGDEAQS KRGILTLKYP IEHGIVTNWD DMEKIWHHTF YNELRVAPEE
HPVLLTEAPL NPKANREKMT QIMFETFNTP AMYVAIQAVL SLYASGRTTG
IVMDSGDGVT HTVPIYEGYA LPHAILRLDL AGRDLTDYLM KILTERGYSF
TTTAEREIVR DIKEKLCYVA LDFEQEMATA ASSSSLEKSY ELPDGQVITI
GNERFRCPEA LFQPSFLGME SCGIHETTFN SIMKCDVDIR KDLYANTVLS
GGTTMYPGIA DRMQKEITAL APSTMKIKII APPERKYSVW IGGSILASLS
TFQQMWISKQ EYDESGPSIV HRKCF

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Human ACTB ELISA Kit

Human Beta-actin ELISA Kit;Human BRWS1 ELISA Kit;Human PS1TP5BP1 ELISA Kit;Human actin beta ELISA Kit;Human actin, cytoplasmic 1 ELISA Kit;Human PS1TP5-binding protein 1 ELISA Kit;Human beta cytoskeletal actin ELISA Kit;