Human LEP ELISA Kit

Alternative name
Human obese protein ELISA Kit;Human obesity factor ELISA Kit;Human LEPD ELISA Kit;Human OB ELISA Kit;Human OBS ELISA Kit;Human leptin ELISA Kit;Human leptin (murine obesity homolog) ELISA Kit;Human leptin (obesity homolog, mouse) ELISA Kit;Human obese, mouse, homolog of ELISA Kit;
Catalog
E022364
species
Human
GeneLEP
SpecificityNo cross-reaction with other factors.
SamplesSerum, Plasma , tissue homogenates,Cell culture supernates,Other biological fluids.
Sensitivity0.05 ng/ml.
Intended UseHuman LEP ELISA Kit allows for the in vitro quantitative determination of LEP , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
StorageStore the whole ELISA kit at 4℃
Product Description
specificalPrinciple of the assay: This experiment use double-sandwich elisa technique and the ELISA Kit provided is typical. The pre-coated antibody is human Lep monoclonal antibody and the detecting antibody is polyclonal antibody with biotin labeled. Samples and biotin labeling antibody are added into ELISA plate wells and washed out with PBS or TBS. Then Avidin-peroxidase conjugates are added to ELISA wells in order; Use TMB substrate for coloring after reactant thoroughly washed out by PBS or TBS. TMB turns into blue in peroxidase catalytic and finally turns into yellow under the action of acid. The color depth and the testing factors in samples are positively correlated.
Human Leptin Protein information
Uniprot ID LEP_HUMAN
Uniprot AC P41159; O15158; Q56A88;
UniGene Hs.194236;
GeneID 3952
KEGG hsa:3952;
Human Leptin Protein SEQUENCE
SEQUENCE 167 AA; 18641 MW; C91A121E92D37B69 CRC64;
MHWGTLCGFL WLWPYLFYVQ AVPIQKVQDD TKTLIKTIVT RINDISHTQS
VSSKQKVTGL DFIPGLHPIL TLSKMDQTLA VYQQILTSMP SRNVIQISND
LENLRDLLHV LAFSKSCHLP WASGLETLDS LGGVLEASGY STEVVALSRL
QGSLQDMLWQ LDLSPGC
UCSC uc003vml.3; human.;

Human LEP ELISA Kit

Human obese protein ELISA Kit;Human obesity factor ELISA Kit;Human LEPD ELISA Kit;Human OB ELISA Kit;Human OBS ELISA Kit;Human leptin ELISA Kit;Human leptin (murine obesity homolog) ELISA Kit;Human leptin (obesity homolog, mouse) ELISA Kit;Human obese, mouse, homolog of ELISA Kit;

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