Human LEP ELISA Kit

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  • Alternative name

    Human obese protein ELISA Kit;Human obesity factor ELISA Kit;Human LEPD ELISA Kit;Human OB ELISA Kit;Human OBS ELISA Kit;Human leptin ELISA Kit;Human leptin (murine obesity homolog) ELISA Kit;Human leptin (obesity homolog, mouse) ELISA Kit;Human obese, mouse, homolog of ELISA Kit;

  • Catalog
    E022364
  • species
    Human
  • GeneLEP
  • Standard CurveHuman LEP ELISA Kit
  • Other Species Mouse Lep ELISA KitMouse Lep IgG ELISA KitBovine LEP ELISA KitCanine LEP ELISA KitGoat LEP ELISA KitChicken LEP ELISA KitHorse LEP ELISA KitTurkey LEP ELISA KitPorcine LEP ELISA KitRat Lep ELISA KitSheep LEP ELISA Kit
  • SpecificityNo cross-reaction with other factors.
  • SamplesSerum, Plasma , tissue homogenates,Cell culture supernates,Other biological fluids.
  • Sensitivity0.05 ng/ml.
  • Intended UseHuman LEP ELISA Kit allows for the in vitro quantitative determination of LEP , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
  • StorageStore the whole ELISA kit at 4℃
  • Product Description
    specifical
    Principle of the assay: This experiment use double-sandwich elisa technique and the ELISA Kit provided is typical. The pre-coated antibody is human Lep monoclonal antibody and the detecting antibody is polyclonal antibody with biotin labeled. Samples and biotin labeling antibody are added into ELISA plate wells and washed out with PBS or TBS. Then Avidin-peroxidase conjugates are added to ELISA wells in order; Use TMB substrate for coloring after reactant thoroughly washed out by PBS or TBS. TMB turns into blue in peroxidase catalytic and finally turns into yellow under the action of acid. The color depth and the testing factors in samples are positively correlated.
  • Human Leptin Protein information
  • Uniprot ID LEP_HUMAN
  • Uniprot AC P41159; O15158; Q56A88;
  • UniGene Hs.194236;
  • GeneID 3952
  • KEGG hsa:3952;
  • Human Leptin Protein SEQUENCE
  • SEQUENCE 167 AA; 18641 MW; C91A121E92D37B69 CRC64;

    MHWGTLCGFL WLWPYLFYVQ AVPIQKVQDD TKTLIKTIVT RINDISHTQS

    VSSKQKVTGL DFIPGLHPIL TLSKMDQTLA VYQQILTSMP SRNVIQISND

    LENLRDLLHV LAFSKSCHLP WASGLETLDS LGGVLEASGY STEVVALSRL

    QGSLQDMLWQ LDLSPGC

  • UCSC uc003vml.3; human.;



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