Human TLR8 ELISA Kit

Alternative name
Human CD288 ELISA Kit;Human toll like receptor 8 ELISA Kit;Human toll-like receptor 8 ELISA Kit;
Catalog
E038544
species
Human
GeneTLR8
SamplesSerum, Plasma , tissue homogenates,Cell culture supernates,Other biological fluids.
Sensitivity0.1 ng/mL.
Intended UseHuman TLR8 ELISA Kit allows for the in vitro quantitative determination of TLR8 , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
StorageStore the whole ELISA kit at 4℃
Product Categories/FamilyImmunology
Product Description
specificalIntended Uses: This TLR-8 ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Human TLR-8. This ELISA kit for research use only, not for therapeutic or diagnostic applications! Principle of the Assay: TLR-8 ELISA kit applies the competitive enzyme immunoassay technique utilizing a monoclonal anti-TLR-8 antibody and an TLR-8-HRP conjugate. The assay sample and buffer are incubated together with TLR-8-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the TLR-8 concentration since TLR-8 from samples and TLR-8-HRP conjugate compete for the anti-TLR-8 antibody binding site. Since the number of sites is limited, as more sites are occupied by TLR-8 from the sample, fewer sites are left to bind TLR-8-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The TLR-8 concentration in each sample is interpolated from this standard curve.
Human Toll-like receptor 8 Protein information
Uniprot ID TLR8_HUMAN
Uniprot AC Q9NR97; B3Y654; D1CS70; D1CS76; Q495P4; Q6UXL6; Q9NYG9;
UniGene Hs.660543;
GeneID 51311
KEGG hsa:51311;
Human Toll-like receptor 8 Protein SEQUENCE
SEQUENCE 1041 AA; 119828 MW; 39A38B60629291C8 CRC64;
MENMFLQSSM LTCIFLLISG SCELCAEENF SRSYPCDEKK QNDSVIAECS
NRRLQEVPQT VGKYVTELDL SDNFITHITN ESFQGLQNLT KINLNHNPNV
QHQNGNPGIQ SNGLNITDGA FLNLKNLREL LLEDNQLPQI PSGLPESLTE
LSLIQNNIYN ITKEGISRLI NLKNLYLAWN CYFNKVCEKT NIEDGVFETL
TNLELLSLSF NSLSHVPPKL PSSLRKLFLS NTQIKYISEE DFKGLINLTL
LDLSGNCPRC FNAPFPCVPC DGGASINIDR FAFQNLTQLR YLNLSSTSLR
KINAAWFKNM PHLKVLDLEF NYLVGEIASG AFLTMLPRLE ILDLSFNYIK
GSYPQHINIS RNFSKLLSLR ALHLRGYVFQ ELREDDFQPL MQLPNLSTIN
LGINFIKQID FKLFQNFSNL EIIYLSENRI SPLVKDTRQS YANSSSFQRH
IRKRRSTDFE FDPHSNFYHF TRPLIKPQCA AYGKALDLSL NSIFFIGPNQ
FENLPDIACL NLSANSNAQV LSGTEFSAIP HVKYLDLTNN RLDFDNASAL
TELSDLEVLD LSYNSHYFRI AGVTHHLEFI QNFTNLKVLN LSHNNIYTLT
DKYNLESKSL VELVFSGNRL DILWNDDDNR YISIFKGLKN LTRLDLSLNR
LKHIPNEAFL NLPASLTELH INDNMLKFFN WTLLQQFPRL ELLDLRGNKL
LFLTDSLSDF TSSLRTLLLS HNRISHLPSG FLSEVSSLKH LDLSSNLLKT
INKSALETKT TTKLSMLELH GNPFECTCDI GDFRRWMDEH LNVKIPRLVD
VICASPGDQR GKSIVSLELT TCVSDVTAVI LFFFTFFITT MVMLAALAHH
LFYWDVWFIY NVCLAKVKGY RSLSTSQTFY DAYISYDTKD ASVTDWVINE
LRYHLEESRD KNVLLCLEER DWDPGLAIID NLMQSINQSK KTVFVLTKKY
AKSWNFKTAF YLALQRLMDE NMDVIIFILL EPVLQHSQYL RLRQRICKSS
ILQWPDNPKA EGLFWQTLRN VVLTENDSRY NNMYVDSIKQ Y
UCSC uc004cvd.4; human. [Q9NR97-1];

Category

Human TLR8 ELISA Kit

Human CD288 ELISA Kit;Human toll like receptor 8 ELISA Kit;Human toll-like receptor 8 ELISA Kit;