Human RAD50 ELISA Kit

Alternative name
Human hRad50 ELISA Kit;Human NBSLD ELISA Kit;Human RAD502 ELISA Kit;Human RAD50 double strand break repair protein ELISA Kit;Human DNA repair protein RAD50 ELISA Kit;Human RAD50 homolog, double strand break repair protein ELISA Kit;
Catalog
E012160
species
Human
GeneRAD50
Standard Curve
Other Species Mouse Rad50 ELISA Kit Rat Rad50 ELISA Kit
SpecificityThis assay has high sensitivity and excellent specificity for detection of DNA Repair Protein RAD50 (RAD50). No significant cross-reactivity or interference between DNA Repair Protein RAD50 (RAD50) and analogues was observed.
SamplesSerum, Plasma , tissue homogenates,Cell culture supernates,Other biological fluids.
SensitivityTypically less than 0.057ng/mL.
Intended UseHuman RAD50 ELISA Kit allows for the in vitro quantitative determination of RAD50 , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
StorageStore the whole ELISA kit at 4℃
Product Description
specificalPrinciple of the Assay: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to DNA Repair Protein RAD50 (RAD50). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to DNA Repair Protein RAD50 (RAD50). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain DNA Repair Protein RAD50 (RAD50), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of DNA Repair Protein RAD50 (RAD50) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
Human DNA repair protein RAD50 Protein information
Uniprot ID RAD50_HUMAN
Uniprot AC Q92878; B9EGF5; O43254; Q6GMT7; Q6P5X3; Q9UP86;
UniGene Hs.633509;
GeneID 10111
KEGG hsa:10111;
Human DNA repair protein RAD50 Protein SEQUENCE
SEQUENCE 1312 AA; 153892 MW; 1F208C3817FC41FC CRC64;
MSRIEKMSIL GVRSFGIEDK DKQIITFFSP LTILVGPNGA GKTTIIECLK
YICTGDFPPG TKGNTFVHDP KVAQETDVRA QIRLQFRDVN GELIAVQRSM
VCTQKSKKTE FKTLEGVITR TKHGEKVSLS SKCAEIDREM ISSLGVSKAV
LNNVIFCHQE DSNWPLSEGK ALKQKFDEIF SATRYIKALE TLRQVRQTQG
QKVKEYQMEL KYLKQYKEKA CEIRDQITSK EAQLTSSKEI VKSYENELDP
LKNRLKEIEH NLSKIMKLDN EIKALDSRKK QMEKDNSELE EKMEKVFQGT
DEQLNDLYHN HQRTVREKER KLVDCHRELE KLNKESRLLN QEKSELLVEQ
GRLQLQADRH QEHIRARDSL IQSLATQLEL DGFERGPFSE RQIKNFHKLV
RERQEGEAKT ANQLMNDFAE KETLKQKQID EIRDKKTGLG RIIELKSEIL
SKKQNELKNV KYELQQLEGS SDRILELDQE LIKAERELSK AEKNSNVETL
KMEVISLQNE KADLDRTLRK LDQEMEQLNH HTTTRTQMEM LTKDKADKDE
QIRKIKSRHS DELTSLLGYF PNKKQLEDWL HSKSKEINQT RDRLAKLNKE
LASSEQNKNH INNELKRKEE QLSSYEDKLF DVCGSQDFES DLDRLKEEIE
KSSKQRAMLA GATAVYSQFI TQLTDENQSC CPVCQRVFQT EAELQEVISD
LQSKLRLAPD KLKSTESELK KKEKRRDEML GLVPMRQSII DLKEKEIPEL
RNKLQNVNRD IQRLKNDIEE QETLLGTIMP EEESAKVCLT DVTIMERFQM
ELKDVERKIA QQAAKLQGID LDRTVQQVNQ EKQEKQHKLD TVSSKIELNR
KLIQDQQEQI QHLKSTTNEL KSEKLQISTN LQRRQQLEEQ TVELSTEVQS
LYREIKDAKE QVSPLETTLE KFQQEKEELI NKKNTSNKIA QDKLNDIKEK
VKNIHGYMKD IENYIQDGKD DYKKQKETEL NKVIAQLSEC EKHKEKINED
MRLMRQDIDT QKIQERWLQD NLTLRKRNEE LKEVEEERKQ HLKEMGQMQV
LQMKSEHQKL EENIDNIKRN HNLALGRQKG YEEEIIHFKK ELREPQFRDA
EEKYREMMIV MRTTELVNKD LDIYYKTLDQ AIMKFHSMKM EEINKIIRDL
WRSTYRGQDI EYIEIRSDAD ENVSASDKRR NYNYRVVMLK GDTALDMRGR
CSAGQKVLAS LIIRLALAET FCLNCGIIAL DEPTTNLDRE NIESLAHALV
EIIKSRSQQR NFQLLVITHD EDFVELLGRS EYVEKFYRIK KNIDQCSEIV
KCSVSSLGFN VH
UCSC uc003kxi.4; human. [Q92878-1];

Category

Human RAD50 ELISA Kit

Human hRad50 ELISA Kit;Human NBSLD ELISA Kit;Human RAD502 ELISA Kit;Human RAD50 double strand break repair protein ELISA Kit;Human DNA repair protein RAD50 ELISA Kit;Human RAD50 homolog, double strand break repair protein ELISA Kit;