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Human TNNT1 ELISA Kit

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  • Alternative name

    Human TNTS ELISA Kit;Human slow skeletal muscle troponin T ELISA Kit;Human STNT ELISA Kit;Human TNT ELISA Kit;Human ANM ELISA Kit;Human NEM5 ELISA Kit;Human troponin T1, slow skeletal type ELISA Kit;Human troponin T, slow skeletal muscle ELISA Kit;Human troponin T type 1 (skeletal, slow) ELISA Kit;Human troponin-T1, skeletal, slow ELISA Kit;
  • Catalog
    E038516
  • species
    Human
  • GeneTNNT1
  • SpecificityThis kit recognizes natural and recombinant Human TNNT1. No significant cross-reactivity or interference between Human TNNT1 and analogues was observed.
  • SamplesSerum, Plasma , tissue homogenates,Cell culture supernates,Other biological fluids.
  • SensitivityThe minimum detectable dose of Human TNNT1 is 9.375pg/mL (The sensitivity of this assay, or lowest detectable limit (LDL) was defined as the lowest protein concentration that could be differentiated from zero).
  • Intended UseHuman TNNT1 ELISA Kit allows for the in vitro quantitative determination of TNNT1 , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
  • StorageStore the whole ELISA kit at 4℃
  • Product Description
    specifical    Principle of the assay: This ELISA kit uses Sandwich-ELISA as the method. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to TNNT1. Standards or samples are added to the appropriate micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for TNNT1 and Avidin-Horseradish Peroxidase (HRP) conjugate is added to each micro plate well successively and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain TNNT1, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm +/- 2 nm. The OD value is proportional to the concentration of TNNT1. You can calculate the concentration of TNNT1 in the samples by comparing the OD of the samples to the standard curve.
  • Human Troponin T, slow skeletal muscle Protein information
  • Uniprot ID TNNT1_HUMAN
  • Uniprot AC P13805; O95472; Q16061; Q5U0E1;
  • UniGene Hs.631558;
  • GeneID 7138
  • KEGG hsa:7138;
  • Human Troponin T, slow skeletal muscle Protein SEQUENCE

  • SEQUENCE 278 AA; 32948 MW; D94DB18BC5804E04 CRC64;

    MSDTEEQEYE EEQPEEEAAE EEEEAPEEPE PVAEPEEERP KPSRPVVPPL

    IPPKIPEGER VDFDDIHRKR MEKDLLELQT LIDVHFEQRK KEEEELVALK

    ERIERRRSER AEQQRFRTEK ERERQAKLAE EKMRKEEEEA KKRAEDDAKK

    KKVLSNMGAH FGGYLVKAEQ KRGKRQTGRE MKVRILSERK KPLDIDYMGE

    EQLRARSAWL PPSQPSCPAR EKAQELSDWI HQLESEKFDL MAKLKQQKYE

    INVLYNRISH AQKFRKGAGK GRVGGRWK

  • UCSC uc002qjb.5; human. [P13805-1];



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