Mouse 5-NT ELISA Kit

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  • Alternative name

    5'-nucleotidase ELISA KIT; Ecto-5'-nucleotidase ELISA KIT; CD_antigen: CD73NT5E ELISA KIT; NT5 ELISA KIT; NTE ELISA KIT; 5'-NT ELISA KIT

  • Catalog
    E073794
  • species
    Mouse
  • Gene5-NT
  • Other Species Human 5-NT ELISA KitHuman NT-5 ELISA Kit
  • SpecificityThis assay has high sensitivity and excellent specificity for detection of mouse 5-NT. No significant cross-reactivity or interference between mouse 5-NT and analogues was observed.
  • SamplesSerum, Plasma , tissue homogenates,Cell culture supernates,Other biological fluids.
  • Sensitivity0.031 mU/ml.
  • Intended UseMouse 5-NT ELISA Kit allows for the in vitro quantitative determination of 5-NT , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
  • StorageFor 5-7days:Store the whole kit at 4℃
    For a Long time :Store the Substrate at 4℃, other reagent should store at -20℃.
  • Product Description
    specifical
    Principle of the Assay||This assay employs the quantitative sandwich enzyme immunoassay technique. Antibody specific for 5-NT has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any 5-NT present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for 5-NT is added to the wells. After washing, avidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of 5-NT bound in the initial step. The color development is stopped and the intensity of the color is measured.


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