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Alternative name
Tumor necrosis factor ligand superfamily member 15 ELISA KIT; TNF ligand-related molecule 1 ELISA KIT; Vascular endothelial cell growth inhibitorCleaved into the following 2 chains:Tumor necrosis factor ligand superfamily member 15, membrane form ELISA KIT; Tumor necrosis factor ligand superfamily member 15, secreted formTNFSF15 ELISA KIT; TL1 ELISA KIT; VEGI ELISA KIT
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Catalog
E038433
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species
Human
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GeneTL1A
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SpecificityThis kit recognizes natural and recombinant Human TL1A. No significant cross-reactivity or interference between Human TL1A and analogues was observed.
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SamplesSerum, Plasma , tissue homogenates,Cell culture supernates,Other biological fluids.
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SensitivityThe minimum detectable dose of Human TL1A is 46.875pg/ml
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Intended UseHuman TL1A ELISA Kit allows for the in vitro quantitative determination of TL1A , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
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StorageFor 5-7days:Store the whole kit at 4℃
For a Long time :Store the Substrate at 4℃, other reagent should store at -20℃.
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Product Description
specificalIntended Uses||This ELISA kit applies to the in vitro quantitative determination of Human TL1A concentrations in serum, plasma and other biological fluids.
Principle of the Assay: This ELISA kit uses Sandwich-ELISA as the method. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to TL1A. Standards or samples are added to the appropriate micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for TL1A and Avidin-Horseradish Peroxidase (HRP) conjugate is added to each micro plate well successively and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain TL1A, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm +/- 2 nm. The OD value is proportional to the concentration of TL1A. You can calculate the concentration of TL1A in the samples by comparing the OD of the samples to the standard curve.
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