Human ANPEP ELISA Kit

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  • Alternative name

    Human AP-N ELISA Kit;Human hAPN ELISA Kit;Human Alanyl aminopeptidase ELISA Kit;Human aminopeptidase M ELISA Kit;Human AP-M ELISA Kit;Human microsomal aminopeptidase ELISA Kit;Human myeloid plasma membrane glycoprotein CD13 ELISA Kit;Human GP150 ELISA Kit;Human APN ELISA Kit;Human CD13 ELISA Kit;Human PEPN ELISA Kit;Human LAP1 ELISA Kit;Human P150 ELISA Kit;Human alanyl aminopeptidase, membrane ELISA Kit;Human aminopeptidase N ELISA Kit;Human alanyl (membrane) aminopeptidase ELISA Kit;Human membrane alanyl aminopeptidase ELISA Kit;

  • Catalog
    E001238
  • species
    Human
  • GeneANPEP
  • Other Species Mouse Anpep ELISA KitBovine ANPEP ELISA KitCanine ANPEP ELISA KitChicken ANPEP ELISA KitPorcine ANPEP ELISA KitRabbit ANPEP ELISA KitRat Anpep ELISA Kit
  • SpecificityThis assay has high sensitivity and excellent specificity for detection of human ANPEP. No significant cross-reactivity or interference between human ANPEP and analogues was observed.
  • SamplesSerum, Plasma , tissue homogenates,Cell culture supernates,Other biological fluids.
  • SensitivityThe minimum detectable dose of human ANPEP is typically less than 7.81 pg/ml. The sensitivity of this assay, or Lower Limit of Detection (LLD) was defined as the lowest protein concentration that could be differentiated from zero. It was determined the me
  • Intended UseHuman ANPEP ELISA Kit allows for the in vitro quantitative determination of ANPEP , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
  • StorageStore the whole ELISA kit at 4℃
  • Product Description
    specifical
    Principle of the assay: This assay employs the quantitative sandwich enzyme immunoassay technique. Antibody specific for ANPEP has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any ANPEP present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for ANPEP is added to the wells. After washing, avidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of ANPEP bound in the initial step. The color development is stopped and the intensity of the color is measured.
  • Human Aminopeptidase N Protein information
  • Uniprot ID AMPN_HUMAN
  • Uniprot AC P15144; Q16728; Q6GT90; Q8IUK3; Q8IVH3; Q9UCE0;
  • UniGene Hs.1239;
  • GeneID 290
  • KEGG hsa:290;
  • Human Aminopeptidase N Protein SEQUENCE
  • SEQUENCE 967 AA; 109540 MW; 37B6BC1BF0D6B1F2 CRC64;

    MAKGFYISKS LGILGILLGV AAVCTIIALS VVYSQEKNKN ANSSPVASTT

    PSASATTNPA SATTLDQSKA WNRYRLPNTL KPDSYRVTLR PYLTPNDRGL

    YVFKGSSTVR FTCKEATDVI IIHSKKLNYT LSQGHRVVLR GVGGSQPPDI

    DKTELVEPTE YLVVHLKGSL VKDSQYEMDS EFEGELADDL AGFYRSEYME

    GNVRKVVATT QMQAADARKS FPCFDEPAMK AEFNITLIHP KDLTALSNML

    PKGPSTPLPE DPNWNVTEFH TTPKMSTYLL AFIVSEFDYV EKQASNGVLI

    RIWARPSAIA AGHGDYALNV TGPILNFFAG HYDTPYPLPK SDQIGLPDFN

    AGAMENWGLV TYRENSLLFD PLSSSSSNKE RVVTVIAHEL AHQWFGNLVT

    IEWWNDLWLN EGFASYVEYL GADYAEPTWN LKDLMVLNDV YRVMAVDALA

    SSHPLSTPAS EINTPAQISE LFDAISYSKG ASVLRMLSSF LSEDVFKQGL

    ASYLHTFAYQ NTIYLNLWDH LQEAVNNRSI QLPTTVRDIM NRWTLQMGFP

    VITVDTSTGT LSQEHFLLDP DSNVTRPSEF NYVWIVPITS IRDGRQQQDY

    WLIDVRAQND LFSTSGNEWV LLNLNVTGYY RVNYDEENWR KIQTQLQRDH

    SAIPVINRAQ IINDAFNLAS AHKVPVTLAL NNTLFLIEER QYMPWEAALS

    SLSYFKLMFD RSEVYGPMKN YLKKQVTPLF IHFRNNTNNW REIPENLMDQ

    YSEVNAISTA CSNGVPECEE MVSGLFKQWM ENPNNNPIHP NLRSTVYCNA

    IAQGGEEEWD FAWEQFRNAT LVNEADKLRA ALACSKELWI LNRYLSYTLN

    PDLIRKQDAT STIISITNNV IGQGLVWDFV QSNWKKLFND YGGGSFSFSN

    LIQAVTRRFS TEYELQQLEQ FKKDNEETGF GSGTRALEQA LEKTKANIKW

    VKENKEVVLQ WFTENSK

  • UCSC uc002bop.5; human.;


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