Human TMSAlpha 1 ELISA Kit

Contact Us
  • Catalog
    E038187
  • species
    Human
  • GeneTMSAlpha 1
  • Standard CurveHuman TMSAlpha 1 ELISA Kit
  • SpecificityThis assay has high sensitivity and excellent specificity for detection of TMSa1. No significant cross-reactivity or interference between TMSa1 and analogues was observed. NOTE: Limited by current skills and knowledge, it is impossible for us to complete the cross-reactivity detection between TMSa1 and all the analogues, therefore, cross reaction may still exist in some cases.
  • SamplesSerum, Plasma , tissue homogenates,Cell culture supernates,Other biological fluids.
  • Sensitivity1.0 ng/mL.
  • Intended UseHuman TMSAlpha 1 ELISA Kit allows for the in vitro quantitative determination of TMSAlpha 1 , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
  • StorageFor 5-7days:Store the whole kit at 4℃
    For a Long time :Store the Substrate at 4℃, other reagent should store at -20℃.
  • Product Categories/FamilySignal Transduction
  • Product Description
    specifical
    Principle of the Assay: TMSa1 ELISA kit applies the competitive enzyme immunoassay technique utilizing a Polyclonal anti-TMSa1 antibody and an TMSa1-HRP conjugate. The assay sample and buffer are incubated together with TMSa1-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the TMSa1 concentration since TMSa1 from samples and TMSa1-HRP conjugate compete for the anti-TMSa1 antibody binding site. Since the number of sites is limited, as more sites are occupied by TMSa1 from the sample, fewer sites are left to bind TMSa1-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The TMSa1 concentration in each sample is interpolated from this standard curve.


Related products with Human TMSAlpha 1 ELISA Kit

Inquiry About Human TMSAlpha 1 ELISA Kit If you hope to order it or contact us directly, please contact us via sales@biobool.com. We will get back to you in 12hrs,Thanks!