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Human SAP25 ELISA Kit

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  • Alternative name

    Human 25 kDa Sin3-associated polypeptide ELISA Kit;Human Sin3 corepressor complex subunit SAP25 ELISA Kit;Human Sin3A associated protein 25 ELISA Kit;Human histone deacetylase complex subunit SAP25 ELISA Kit;Human Sin3A-associated protein, 25kDa ELISA Kit;Human sin3-associated polypeptide, 25kDa ELISA Kit;Human sin3A-binding protein 25 ELISA Kit;
  • Catalog
    E034222
  • species
    Human
  • GeneSAP25
  • SpecificityThis assay has high sensitivity and excellent specificity for detection of Human SAP25. No significant cross-reactivity or interference between Human SAP25 and analogues was observed. Note: Limited by current skills and knowledge, it is impossible for us to complete the cross- reactivity detection between Human SAP25 and all the analogues, therefore, cross reaction may still exist.
  • SamplesSerum, Plasma , tissue homogenates,Cell culture supernates,Other biological fluids.
  • Intended UseHuman SAP25 ELISA Kit allows for the in vitro quantitative determination of SAP25 , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
  • StorageStore the whole ELISA kit at 4℃
  • Product Description
    specifical    Description: The kit is a sandwich enzyme immunoassay for in vitro quantitative measurement of SAP25 in human serum, plasma and other biological fluids Principle of the Assay: This ELISA kit uses Sandwich-ELISA as the method. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Human SAP25. Standards or samples are added to the appropriate micro ELISA plate wells and bound by the specific antibody. Then a biotinylated detection antibody specific for Human SAP25 and Avidin-Horseradish Peroxidase (HRP) conjugate is added to each micro plate well successively and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Human SAP25, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm +/- 2 nm. The OD value is proportional to the concentration of Human SAP25. You can calculate the concentration of Human SAP25 in the samples by comparing the OD of the samples to the standard curve.
  • Human Histone deacetylase complex subunit SAP25 Protein information
  • Uniprot ID SAP25_HUMAN
  • Uniprot AC Q8TEE9;
  • UniGene Hs.633863;
  • GeneID 100316904
  • KEGG hsa:100316904;
  • Human Histone deacetylase complex subunit SAP25 Protein SEQUENCE

  • SEQUENCE 199 AA; 20873 MW; F0ADC8A992B40BE2 CRC64;

    MTPLAPWDPK YEAKAGPRPV WGANCSSGAS FSGRTLCHPS FWPLYEAASG

    RGLRPVAPAT GHWNGQQAPP DAGFPVVCCE DVFLSDPLLP RGQRVPLYLS

    KAPQQMMGSL KLLPPPPIMS ARVLPRPSPS RGPSTAWLSG PELIALTGLL

    QMSQGEPRPS SSAVGPPDHT SDPPSPCGSP SSSQGADLSL PQTPDTHCP

  • UCSC uc022aip.2; human.;



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