Intended UseMouse Sirt1O ELISA Kit allows for the in vitro quantitative determination of Sirt1O , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
StorageFor 5-7days:Store the whole kit at 4℃
For a Long time :Store the Substrate at 4℃, other reagent should store at -20℃.
Product Description specificalPrinciple of the Assay: The microtiter plate provided in this kit has been pre-coated with an antibody specific to NAD-dependent deacetylase sirtuin-1. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated polyclonal antibody preparation specific for NAD-dependent deacetylase sirtuin-1 and Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. Only those wells that contain NAD-dependent deacetylase sirtuin-1, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm +/- 2 nm. The concentration of NAD-dependent deacetylase sirtuin-1 in the samples is then determined by comparing the O.D. of the samples to the standard curve.